-
Something wrong with this record ?
Virulence factors genes in enterococci isolated from beavers (Castor fiber)
A. Lauková, V. Strompfová, A. Kandričáková, J. Ščerbová, T. Semedo-Lemsaddek, R. Miltko, G. Belzecki,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacteriological Techniques MeSH
- Enterococcus classification drug effects genetics isolation & purification MeSH
- Virulence Factors analysis genetics MeSH
- Feces microbiology MeSH
- Genotype MeSH
- Gram-Positive Bacterial Infections microbiology veterinary MeSH
- Rodentia microbiology MeSH
- Microbial Sensitivity Tests MeSH
- Polymerase Chain Reaction MeSH
- Rectum microbiology MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Gelatinases analysis MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Poland MeSH
Only limited information exists concerning the microbiota in beaver (Castor fiber). This study has been focused on the virulence factors genes detection in enterococci from beavers. In general, animals are not affected by enterococcal infections, but they can be a reservoir of, e.g. pathogenic strains. Moreover, detection of virulence factors genes in enterococci from beavers was never tested before. Free-living beavers (12), male and female (age 4-5 years) were caught in the north-east part of Poland. Sampling of lower gut and faeces was provided according to all ethical rules for animal handling. Samples were treated using a standard microbiological method. Pure bacterial colonies were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) identification system. Virulence factors genes-gelE (gelatinase), agg (aggregation), cylA (cytolysin A), efaAfs (adhesin Enterococcus faecalis), efaAfm (adhesin Enterococcus faecium) and esp (surface protein) were tested by PCR. Moreover, gelatinase and antibiotic phenotypes were tested. Species detected were Enterococcus thailandicus, E. faecium, E. faecalis and Enterococcus durans. In literature, enterococcal species distribution was never reported yet up to now. Strains were mostly sensitive to antibiotics. Vancomycin-resistant E. faecalis EE9Tr1 possess cylA, efaAfs, esp and gelE genes. Strains were aggregation substance genes absent. Adhesin E. faecium (efaAfm) gene was detected in two of three E. faecium strains, but it was present also in E. thailandicus. Esp gene was present in EE9Tr1 and E. durans EDTr92. The most detected were gelE, efaAfm genes; in EF 4Hc1 also gelatinase phenotype was found. Strains with virulence factors genes will be tested for their sensitivity to antimicrobial enterocins.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15036708
- 003
- CZ-PrNML
- 005
- 20211105133442.0
- 007
- ta
- 008
- 151120s2015 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-014-0357-8 $2 doi
- 035 __
- $a (PubMed)25300356
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Lauková, Andrea $u Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia, laukova@saske.sk.
- 245 10
- $a Virulence factors genes in enterococci isolated from beavers (Castor fiber) / $c A. Lauková, V. Strompfová, A. Kandričáková, J. Ščerbová, T. Semedo-Lemsaddek, R. Miltko, G. Belzecki,
- 520 9_
- $a Only limited information exists concerning the microbiota in beaver (Castor fiber). This study has been focused on the virulence factors genes detection in enterococci from beavers. In general, animals are not affected by enterococcal infections, but they can be a reservoir of, e.g. pathogenic strains. Moreover, detection of virulence factors genes in enterococci from beavers was never tested before. Free-living beavers (12), male and female (age 4-5 years) were caught in the north-east part of Poland. Sampling of lower gut and faeces was provided according to all ethical rules for animal handling. Samples were treated using a standard microbiological method. Pure bacterial colonies were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) identification system. Virulence factors genes-gelE (gelatinase), agg (aggregation), cylA (cytolysin A), efaAfs (adhesin Enterococcus faecalis), efaAfm (adhesin Enterococcus faecium) and esp (surface protein) were tested by PCR. Moreover, gelatinase and antibiotic phenotypes were tested. Species detected were Enterococcus thailandicus, E. faecium, E. faecalis and Enterococcus durans. In literature, enterococcal species distribution was never reported yet up to now. Strains were mostly sensitive to antibiotics. Vancomycin-resistant E. faecalis EE9Tr1 possess cylA, efaAfs, esp and gelE genes. Strains were aggregation substance genes absent. Adhesin E. faecium (efaAfm) gene was detected in two of three E. faecium strains, but it was present also in E. thailandicus. Esp gene was present in EE9Tr1 and E. durans EDTr92. The most detected were gelE, efaAfm genes; in EF 4Hc1 also gelatinase phenotype was found. Strains with virulence factors genes will be tested for their sensitivity to antimicrobial enterocins.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a antibakteriální látky $x farmakologie $7 D000900
- 650 _2
- $a bakteriologické techniky $7 D001431
- 650 _2
- $a Enterococcus $x klasifikace $x účinky léků $x genetika $x izolace a purifikace $7 D016983
- 650 _2
- $a feces $x mikrobiologie $7 D005243
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a želatinasy $x analýza $7 D018093
- 650 _2
- $a genotyp $7 D005838
- 650 _2
- $a grampozitivní bakteriální infekce $x mikrobiologie $x veterinární $7 D016908
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a mikrobiální testy citlivosti $7 D008826
- 650 _2
- $a polymerázová řetězová reakce $7 D016133
- 650 _2
- $a rektum $x mikrobiologie $7 D012007
- 650 _2
- $a hlodavci $x mikrobiologie $7 D012377
- 650 _2
- $a spektrometrie hmotnostní - ionizace laserem za účasti matrice $7 D019032
- 650 _2
- $a faktory virulence $x analýza $x genetika $7 D037521
- 651 _2
- $a Polsko $7 D011044
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Strompfová, Viola
- 700 1_
- $a Kandričáková, Anna
- 700 1_
- $a Ščerbová, Jana
- 700 1_
- $a Semedo-Lemsaddek, Teresa
- 700 1_
- $a Miltko, Renata
- 700 1_
- $a Belzecki, Grzegorz
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 60, č. 2 (2015), s. 151-4
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25300356 $y Pubmed
- 910 __
- $a ABA008 $b online $c sign $y 4 $z 0
- 990 __
- $a 20151120 $b ABA008
- 991 __
- $a 20211105133447 $b ABA008
- 999 __
- $a ok $b bmc $g 1097898 $s 919895
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 60 $c 2 $d 151-4 $e 20141010 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20151120