-
Je něco špatně v tomto záznamu ?
Chromium speciation and biochemical changes vary in relation to plant ploidy
J. Kováčik, B. Klejdus, P. Babula, ME. Soares, J. Hedbavny, M. de Lourdes Bastos,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- chrom klasifikace metabolismus MeSH
- oxidační stres MeSH
- ploidie * MeSH
- rostlinné geny MeSH
- rostliny enzymologie genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Uptake of trivalent chromium (Cr(III)-chloride), Cr speciation and consequences for the metabolism in chamomile plants with two ploidy levels have been studied. Depletion of fresh biomass, tissue water content and soluble proteins in response to high (120 μM) Cr(III) was ploidy-independent. Cr mainly accumulated in the roots (only negligibly in the shoots) and total root Cr amount was higher in tetraploid ones including the proof with specific fluorescent indicator (naphthalimide-rhodamine) of Cr(III). Quantification of Cr(VI) detected its higher content in tetraploid roots (up to 4.2% from total Cr), indicating partial oxidation of applied Cr(III). Higher H2O2 presence but lower activities of peroxidases were observed in tetraploid roots while nitric oxide, superoxide dismutase and glutathione reductase activities did not differ extensively. Soluble phenols, lignin, non-protein thiols, individual thiols (glutathione and phytochelatin 2) and ascorbic acid responded to high Cr(III) similarly in both cultivars while decrease of minerals was more pronounced in tetraploid ones. It seems that Cr(III)-induced oxidative stress arises from high root Cr uptake and Cr(VI) presence and is related to depletion of thiols. Assay of Krebs cycle acids confirmed rather depletion under 120 μM Cr(III) in both cultivars but increase in citric acid may indicate its involvement in root Cr chelation. Subsequent comparison of Cr(III)-chloride and Cr(III)-nitrate showed similar influence on Cr accumulation and majority of biochemical responses while different impact on phytochelatin 2 amount was the most distinct feature.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16000332
- 003
- CZ-PrNML
- 005
- 20160126101039.0
- 007
- ta
- 008
- 160108s2015 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jinorgbio.2015.01.003 $2 doi
- 035 __
- $a (PubMed)25637829
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Kováčik, Jozef $u Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic; CEITEC - Central European Institute of Technology, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic. Electronic address: jozkovacik@yahoo.com.
- 245 10
- $a Chromium speciation and biochemical changes vary in relation to plant ploidy / $c J. Kováčik, B. Klejdus, P. Babula, ME. Soares, J. Hedbavny, M. de Lourdes Bastos,
- 520 9_
- $a Uptake of trivalent chromium (Cr(III)-chloride), Cr speciation and consequences for the metabolism in chamomile plants with two ploidy levels have been studied. Depletion of fresh biomass, tissue water content and soluble proteins in response to high (120 μM) Cr(III) was ploidy-independent. Cr mainly accumulated in the roots (only negligibly in the shoots) and total root Cr amount was higher in tetraploid ones including the proof with specific fluorescent indicator (naphthalimide-rhodamine) of Cr(III). Quantification of Cr(VI) detected its higher content in tetraploid roots (up to 4.2% from total Cr), indicating partial oxidation of applied Cr(III). Higher H2O2 presence but lower activities of peroxidases were observed in tetraploid roots while nitric oxide, superoxide dismutase and glutathione reductase activities did not differ extensively. Soluble phenols, lignin, non-protein thiols, individual thiols (glutathione and phytochelatin 2) and ascorbic acid responded to high Cr(III) similarly in both cultivars while decrease of minerals was more pronounced in tetraploid ones. It seems that Cr(III)-induced oxidative stress arises from high root Cr uptake and Cr(VI) presence and is related to depletion of thiols. Assay of Krebs cycle acids confirmed rather depletion under 120 μM Cr(III) in both cultivars but increase in citric acid may indicate its involvement in root Cr chelation. Subsequent comparison of Cr(III)-chloride and Cr(III)-nitrate showed similar influence on Cr accumulation and majority of biochemical responses while different impact on phytochelatin 2 amount was the most distinct feature.
- 650 _2
- $a chrom $x klasifikace $x metabolismus $7 D002857
- 650 _2
- $a rostlinné geny $7 D017343
- 650 _2
- $a oxidační stres $7 D018384
- 650 _2
- $a rostliny $x enzymologie $x genetika $x metabolismus $7 D010944
- 650 12
- $a ploidie $7 D011003
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Klejdus, Bořivoj $u Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic; CEITEC - Central European Institute of Technology, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
- 700 1_
- $a Babula, Petr $u Department of Physiology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic.
- 700 1_
- $a Soares, Maria Elisa $u REQUIMTE, Department of Biological Sciences, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
- 700 1_
- $a Hedbavny, Josef $u Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
- 700 1_
- $a de Lourdes Bastos, Maria $u REQUIMTE, Department of Biological Sciences, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
- 773 0_
- $w MED00006646 $t Journal of inorganic biochemistry $x 1873-3344 $g Roč. 145, č. - (2015), s. 70-8
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25637829 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160108 $b ABA008
- 991 __
- $a 20160126101202 $b ABA008
- 999 __
- $a ok $b bmc $g 1102613 $s 924538
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 145 $c - $d 70-8 $e 20150117 $i 1873-3344 $m Journal of inorganic biochemistry $n J Inorg Biochem $x MED00006646
- LZP __
- $a Pubmed-20160108
