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Analysis of B-cell subpopulations in monoclonal gammopathies
P. Všianská, L. Říhová, T. Varmužová, R. Suská, F. Kryukov, A. Mikulášová, R. Kupská, M. Penka, L. Pour, Z. Adam, R. Hájek,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT12425
MZ0
CEP - Centrální evidence projektů
- MeSH
- antigeny povrchové metabolismus MeSH
- chromozomální aberace MeSH
- diferenciální diagnóza MeSH
- dospělí MeSH
- fenotyp MeSH
- imunofenotypizace MeSH
- lidé středního věku MeSH
- lidé MeSH
- mnohočetný myelom diagnóza genetika metabolismus MeSH
- monoklonální gamapatie nejasného významu diagnóza genetika metabolismus MeSH
- paraproteinemie diagnóza genetika metabolismus MeSH
- podskupiny B-lymfocytů metabolismus patologie MeSH
- průtoková cytometrie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Multiple myeloma (MM) is characterized by accumulation of pathological plasma cells (PCs) in bone marrow (BM) as a result of deregulation of B-cell development. To clarify its pathophysiology it is necessary to investigate in detail the developmental stages of B-cells. MATERIALS AND METHODS: Enumeration of total CD19-positive (CD19(+)) cells and their subpopulations together with PCs was done in peripheral blood (PB) and BM of newly diagnosed monoclonal gammopathy patients and control subjects. Representation of subsets was compared among groups and relationships between subset percentage and cytogenetic/biochemical findings were analyzed. RESULTS: A lower number of total CD19(+) cells was found in MM, particularly in advanced stages of disease. Reduction of naive (P < .01) and transitional B-cells (P < .05) and increase of switched memory and switched CD27(-) B-cells and germinal center founder cells were detected in PB of MM compared with controls (P < .01). Similar results were found in BM. β2 microglobulin level in MM positively correlated with the number of PCs and negatively with percentage of naive B-cells (P < .05). CONCLUSION: Our results provided a detailed phenotypic profile and enumeration of B and PC subpopulations in monoclonal gammopathy patients. A reduced number of B-cells and particularly a differentiation shift to more numerous antigen-stimulated forms was observed in MM. This might indicate a potential source of myeloma-initiating cells in one of these subpopulations.
Department of Clinical Haematology University Hospital Brno Brno Czech Republic
Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic
Department of Haematooncology University Hospital Ostrava Ostrava Czech Republic
Faculty of Medicine University of Ostrava Ostrava Czech Republic
Citace poskytuje Crossref.org
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- $a Všianská, Pavla $u Department of Clinical Haematology, University Hospital Brno, Brno, Czech Republic; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. $7 xx0193933
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- $a Analysis of B-cell subpopulations in monoclonal gammopathies / $c P. Všianská, L. Říhová, T. Varmužová, R. Suská, F. Kryukov, A. Mikulášová, R. Kupská, M. Penka, L. Pour, Z. Adam, R. Hájek,
- 520 9_
- $a BACKGROUND: Multiple myeloma (MM) is characterized by accumulation of pathological plasma cells (PCs) in bone marrow (BM) as a result of deregulation of B-cell development. To clarify its pathophysiology it is necessary to investigate in detail the developmental stages of B-cells. MATERIALS AND METHODS: Enumeration of total CD19-positive (CD19(+)) cells and their subpopulations together with PCs was done in peripheral blood (PB) and BM of newly diagnosed monoclonal gammopathy patients and control subjects. Representation of subsets was compared among groups and relationships between subset percentage and cytogenetic/biochemical findings were analyzed. RESULTS: A lower number of total CD19(+) cells was found in MM, particularly in advanced stages of disease. Reduction of naive (P < .01) and transitional B-cells (P < .05) and increase of switched memory and switched CD27(-) B-cells and germinal center founder cells were detected in PB of MM compared with controls (P < .01). Similar results were found in BM. β2 microglobulin level in MM positively correlated with the number of PCs and negatively with percentage of naive B-cells (P < .05). CONCLUSION: Our results provided a detailed phenotypic profile and enumeration of B and PC subpopulations in monoclonal gammopathy patients. A reduced number of B-cells and particularly a differentiation shift to more numerous antigen-stimulated forms was observed in MM. This might indicate a potential source of myeloma-initiating cells in one of these subpopulations.
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- $a Říhová, Lucie $u Department of Clinical Haematology, University Hospital Brno, Brno, Czech Republic; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. Electronic address: lucie.rihova@fnbrno.cz. $7 xx0081970
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- $a Varmužová, Tamara $u Department of Clinical Haematology, University Hospital Brno, Brno, Czech Republic; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. $7 xx0200321
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- $a Suská, Renata $u Department of Clinical Haematology, University Hospital Brno, Brno, Czech Republic. $7 xx0144860
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- $a Kryukov, Fedor $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic; Department of Haematooncology, University Hospital Ostrava, Ostrava, Czech Republic. $7 xx0128646
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- $a Mikulášová, Aneta $u Department of Clinical Haematology, University Hospital Brno, Brno, Czech Republic; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. $7 _AN050306
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- $a Kupská, Renata $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. $7 xx0128684
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- $a Pour, Luděk $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Department of Internal Medicine, Haematology and Oncology, University Hospital Brno, Brno, Czech Republic. $7 xx0102556
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- $a Adam, Zdeněk, $u Department of Internal Medicine, Haematology and Oncology, University Hospital Brno, Brno, Czech Republic. $d 1953- $7 jn19981000018
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- $a Hájek, Roman $u Department of Clinical Haematology, University Hospital Brno, Brno, Czech Republic; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic; Department of Haematooncology, University Hospital Ostrava, Ostrava, Czech Republic.
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