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The impact of standard chemotherapy on miRNA signature in plasma in AML patients
L. Koutova, M. Sterbova, E. Pazourkova, S. Pospisilova, I. Svobodova, A. Horinek, D. Lysak, M. Korabecna,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem, validační studie
Perzistentní odkaz
https://www.medvik.cz/link/bmc16009975
- MeSH
- akutní myeloidní leukemie krev farmakoterapie genetika MeSH
- cytarabin aplikace a dávkování MeSH
- dospělí MeSH
- idarubicin aplikace a dávkování MeSH
- indukce remise MeSH
- konsolidační chemoterapie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA biosyntéza krev genetika MeSH
- mitoxantron aplikace a dávkování MeSH
- pilotní projekty MeSH
- protokoly protinádorové kombinované chemoterapie farmakologie terapeutické užití MeSH
- regulace genové exprese u leukemie účinky léků MeSH
- RNA nádorová biosyntéza krev genetika MeSH
- transkriptom * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- validační studie MeSH
AIM: In our pilot study, we used plasma samples as liquid biopsy to search for miRNA signatures in patients with acute myeloid leukemia (AML) at diagnosis and in remission achieved after standard chemotherapy before planned transplantation. MATERIAL AND METHODS: We examined 10 plasma samples from healthy volunteers and 8 paired samples from patients with AML at diagnosis and in remission using TaqMan MicroRNA Arrays. The results were validated using single-target qPCR reactions run in triplicates. RESULTS: We selected 6 miRNAs with expressions significantly sensitive to therapy: miR-199b-5p, miR-301b, miR-326, miR-361-5p, miR-625 and miR-655. All selected miRNAs were not or very weakly expressed in healthy individuals. They were abundant in plasma in patients at diagnosis but their levels decreased after chemotherapy. CONCLUSION: We detected a therapy sensitive miRNA signature in plasma of patients with AML.
Citace poskytuje Crossref.org
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- $a 10.1016/j.leukres.2015.08.009 $2 doi
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- $a Koutova, Linda $u Department of Hematoonocology, Charles University Hospital in Pilsen, Alej Svobody 80, 304 60 Pilsen, Czech Republic.
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- $a The impact of standard chemotherapy on miRNA signature in plasma in AML patients / $c L. Koutova, M. Sterbova, E. Pazourkova, S. Pospisilova, I. Svobodova, A. Horinek, D. Lysak, M. Korabecna,
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- $a AIM: In our pilot study, we used plasma samples as liquid biopsy to search for miRNA signatures in patients with acute myeloid leukemia (AML) at diagnosis and in remission achieved after standard chemotherapy before planned transplantation. MATERIAL AND METHODS: We examined 10 plasma samples from healthy volunteers and 8 paired samples from patients with AML at diagnosis and in remission using TaqMan MicroRNA Arrays. The results were validated using single-target qPCR reactions run in triplicates. RESULTS: We selected 6 miRNAs with expressions significantly sensitive to therapy: miR-199b-5p, miR-301b, miR-326, miR-361-5p, miR-625 and miR-655. All selected miRNAs were not or very weakly expressed in healthy individuals. They were abundant in plasma in patients at diagnosis but their levels decreased after chemotherapy. CONCLUSION: We detected a therapy sensitive miRNA signature in plasma of patients with AML.
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- $a Sterbova, Monika $u Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University in Prague and General Faculty Hospital in Prague, Albertov 4, 128 00 Prague, Czech Republic.
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- $a Pospisilova, Sarka $u Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University in Prague and General Faculty Hospital in Prague, Albertov 4, 128 00 Prague, Czech Republic.
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- $a Korabecna, Marie $u Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University in Prague and General Faculty Hospital in Prague, Albertov 4, 128 00 Prague, Czech Republic; Medical Faculty in Pilsen, Charles University in Prague, Husova 3, 306 05 Pilsen, Czech Republic. Electronic address: marie.korabecna@lf1.cuni.cz.
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