Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Antimicrobial Properties of Microparticles Based on Carmellose Cross-Linked by Cu(2+) Ions

M. Kejdušová, J. Vysloužil, K. Kubová, V. Celer, M. Krásna, A. Pechová, V. Vyskočilová, V. Košťál,

. 2015 ; 2015 (-) : 790720. [pub] 20150519

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc16010091

Carmellose (CMC) is frequently used due to its high biocompatibility, biodegradability, and low immunogenicity for development of site-specific or controlled release drug delivery systems. In this experimental work, CMC dispersions in two different concentrations (1% and 2%) cross-linked by copper (II) ions (0.5, 1, 1.5, or 2.0 M CuCl₂) were used to prepare microspheres with antimicrobial activity against Escherichia coli and Candida albicans, both frequently occurring pathogens which cause vaginal infections. The microparticles were prepared by an ionotropic gelation technique which offers the unique possibility to entrap divalent copper ions in a CMC structure and thus ensure their antibacterial activity. Prepared CMC microspheres exhibited sufficient sphericity. Both equivalent diameter and copper content were influenced by CMC concentration, and the molarity of copper (II) solution affected only the copper content results. Selected samples exhibited stable but pH-responsive behaviour in environments which corresponded with natural (pH 4.5) and inflamed (pH 6.0) vaginal conditions. All the tested samples exhibited proven substantial antimicrobial activity against both Gram-negative bacteria Escherichia coli and yeast Candida albicans. Unexpectedly, a crucial parameter for microsphere antimicrobial activity was not found in the copper content but in the swelling capacity of the microparticles and in the degree of CMC surface shrinking.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16010091
003      
CZ-PrNML
005      
20160408112245.0
007      
ta
008      
160408s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1155/2015/790720 $2 doi
024    7_
$a 10.1155/2015/790720 $2 doi
035    __
$a (PubMed)26090444
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kejdušová, Martina $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
245    10
$a Antimicrobial Properties of Microparticles Based on Carmellose Cross-Linked by Cu(2+) Ions / $c M. Kejdušová, J. Vysloužil, K. Kubová, V. Celer, M. Krásna, A. Pechová, V. Vyskočilová, V. Košťál,
520    9_
$a Carmellose (CMC) is frequently used due to its high biocompatibility, biodegradability, and low immunogenicity for development of site-specific or controlled release drug delivery systems. In this experimental work, CMC dispersions in two different concentrations (1% and 2%) cross-linked by copper (II) ions (0.5, 1, 1.5, or 2.0 M CuCl₂) were used to prepare microspheres with antimicrobial activity against Escherichia coli and Candida albicans, both frequently occurring pathogens which cause vaginal infections. The microparticles were prepared by an ionotropic gelation technique which offers the unique possibility to entrap divalent copper ions in a CMC structure and thus ensure their antibacterial activity. Prepared CMC microspheres exhibited sufficient sphericity. Both equivalent diameter and copper content were influenced by CMC concentration, and the molarity of copper (II) solution affected only the copper content results. Selected samples exhibited stable but pH-responsive behaviour in environments which corresponded with natural (pH 4.5) and inflamed (pH 6.0) vaginal conditions. All the tested samples exhibited proven substantial antimicrobial activity against both Gram-negative bacteria Escherichia coli and yeast Candida albicans. Unexpectedly, a crucial parameter for microsphere antimicrobial activity was not found in the copper content but in the swelling capacity of the microparticles and in the degree of CMC surface shrinking.
650    _2
$a antibakteriální látky $x chemie $x farmakologie $7 D000900
650    _2
$a Candida albicans $x účinky léků $x patogenita $7 D002176
650    _2
$a sodná sůl karboxymethylcelulosy $x chemie $x farmakologie $7 D002266
650    _2
$a měď $x chemie $7 D003300
650    _2
$a léky s prodlouženým účinkem $7 D003692
650    12
$a lékové transportní systémy $7 D016503
650    _2
$a Escherichia coli $x účinky léků $x patogenita $7 D004926
650    _2
$a lidé $7 D006801
650    _2
$a mikrobiální testy citlivosti $7 D008826
650    _2
$a mikrosféry $7 D008863
655    _2
$a časopisecké články $7 D016428
700    1_
$a Vysloužil, Jakub $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
700    1_
$a Kubová, Kateřina $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic.
700    1_
$a Celer, Vladimír $u Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42 Brno, Czech Republic.
700    1_
$a Krásna, Magdaléna $u Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42 Brno, Czech Republic.
700    1_
$a Pechová, Alena $u Department of Biochemistry and Biophysics, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42 Brno, Czech Republic.
700    1_
$a Vyskočilová, Věra $u Department of Biochemistry and Biophysics, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42 Brno, Czech Republic.
700    1_
$a Košťál, Vratislav $u Tescan, Libušina Třída 863/21, 623 00 Brno-Kohoutovice, Czech Republic.
773    0_
$w MED00182164 $t BioMed research international $x 2314-6141 $g Roč. 2015, č. - (2015), s. 790720
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26090444 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160408 $b ABA008
991    __
$a 20160408112323 $b ABA008
999    __
$a ok $b bmc $g 1113520 $s 934459
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 2015 $c - $d 790720 $e 20150519 $i 2314-6141 $m BioMed research international $n Biomed Res Int $x MED00182164
LZP    __
$a Pubmed-20160408

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace