Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers
Language English Country Great Britain, England Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Ascomycota classification genetics isolation & purification MeSH
- DNA Primers genetics MeSH
- Molecular Sequence Data MeSH
- Mycorrhizae classification genetics isolation & purification MeSH
- Polymerase Chain Reaction instrumentation methods MeSH
- Soil Microbiology * MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Primers MeSH
Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field.
References provided by Crossref.org
Nutrient-dependent cross-kingdom interactions in the hyphosphere of an arbuscular mycorrhizal fungus
Predicted climate change will increase the truffle cultivation potential in central Europe
Truffle biogeography-A case study revealing ecological niche separation of different Tuber species
Fine-scale genetic structure of natural Tuber aestivum sites in southern Germany
Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach
