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Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus
J. Felsberg, M. Jelínková, P. Kubizniaková, D. Matoulková,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anaerobiosis MeSH
- Bacteria, Anaerobic classification genetics metabolism MeSH
- DNA, Intergenic chemistry genetics MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- RNA, Ribosomal chemistry genetics MeSH
- Base Sequence MeSH
- Sequence Alignment MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
PCR-primers were designed for identification of strictly anaerobic bacteria of the genus Zymophilus based on genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus 100% specificity. PCR assay developed in this study allows an extension of the spectra of detected beer spoilage microorganisms in brewery laboratories.
References provided by Crossref.org
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- $a PCR-primers were designed for identification of strictly anaerobic bacteria of the genus Zymophilus based on genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus 100% specificity. PCR assay developed in this study allows an extension of the spectra of detected beer spoilage microorganisms in brewery laboratories.
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- $a Kubizniaková, Petra $u Department of Microbiology, Research Institute of Brewing and Malting, Lípová 15, 120 44 Prague, Czech Republic. Electronic address: kubizniakova@beerresearch.cz.
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