Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25725268
DOI
10.1016/j.anaerobe.2015.02.004
PII: S1075-9964(15)00025-6
Knihovny.cz E-resources
- Keywords
- Brewing microbiology, PCR, Strictly anaerobic bacteria, Yeast contamination, Zymophilus paucivorans, Zymophilus raffinosivorans,
- MeSH
- Anaerobiosis MeSH
- Bacteria, Anaerobic classification genetics metabolism MeSH
- DNA, Intergenic chemistry genetics MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- RNA, Ribosomal chemistry genetics MeSH
- Base Sequence MeSH
- Sequence Alignment MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Intergenic MeSH
- RNA, Ribosomal MeSH
PCR-primers were designed for identification of strictly anaerobic bacteria of the genus Zymophilus based on genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus 100% specificity. PCR assay developed in this study allows an extension of the spectra of detected beer spoilage microorganisms in brewery laboratories.
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