• Je něco špatně v tomto záznamu ?

HPLC stanovenie oxidačného poškodenia DNA v perfuzátoch pečene potkanov vplyvom prídavku medi
[HPLC determination of oxidative DNA damage in rat liver perfusates influenced by addition of copper]

Csila Mišľanová, Anton Kebis, Jana Príbojová, Martina Valachovičová

. 2016 ; 110 (1) : 53-58.

Jazyk slovenština Země Česko

Typ dokumentu práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16015092

HPLC determination of a background level of 8 oxodG and 2-dG in rat liver after addition of copper to perfusate was developed. The reversed phase analytical column Purospher® STAR C18e with 50 mmol L–1 phosphate buffer, pH 5.5 and methanol (92:8, v/v) mobile phase was applied for the analysis. The validation of the HPLC method according to linearity, accuracy and precision was carried out. Oxidative DNA damage (expressed as concentration ratio of 8-oxodG/106 2-dG) was determined by the simultaneous measuring of 2-dG with UV detection followed by coulochemical detection of 8 oxodG. The procedure using a model of liver damage caused by intoxication with copper and ischemia / reperfusion with addition of various concentrations of CuSO4 to the perfused rat livers was tested. The aim of this study was to decide whether the toxicity of copper in liver perfusates is related to protein oxidation and oxidative DNA damage. The high contribution to the DNA damage can be related to the physical liver manipulation during harvest and reperfusion as well as to artefacts induced during the sample preparation (time-consuming sample handling during DNA isolation and extraction). The obtained results pointed out that the DNA damage occurred already during liver handling even before application of CuSO4, whereby concentration of CuSO4 higher than 0.03 mmol L–1 caused a total liver damage, which led to a complete stop of the flow of the perfusate.

HPLC determination of oxidative DNA damage in rat liver perfusates influenced by addition of copper

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc16015092
003      
CZ-PrNML
005      
20180530073249.0
007      
ta
008      
160523s2016 xr f 000 0|slo||
009      
AR
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a slo $b eng
044    __
$a xr
100    1_
$a Mišľanová, Csilla $7 xx0261457 $u Fakulta ošetrovateľstva a zdravotnických odborných štúdií, Slovenská zdravotnícka univerzita v Bratislave; Katedra analytickej chémie, Prírodovedecká fakulta, Univerzita Komenského, Bratislava
245    10
$a HPLC stanovenie oxidačného poškodenia DNA v perfuzátoch pečene potkanov vplyvom prídavku medi / $c Csila Mišľanová, Anton Kebis, Jana Príbojová, Martina Valachovičová
246    31
$a HPLC determination of oxidative DNA damage in rat liver perfusates influenced by addition of copper
504    __
$a Literatura
520    3_
$a HPLC determination of a background level of 8 oxodG and 2-dG in rat liver after addition of copper to perfusate was developed. The reversed phase analytical column Purospher® STAR C18e with 50 mmol L–1 phosphate buffer, pH 5.5 and methanol (92:8, v/v) mobile phase was applied for the analysis. The validation of the HPLC method according to linearity, accuracy and precision was carried out. Oxidative DNA damage (expressed as concentration ratio of 8-oxodG/106 2-dG) was determined by the simultaneous measuring of 2-dG with UV detection followed by coulochemical detection of 8 oxodG. The procedure using a model of liver damage caused by intoxication with copper and ischemia / reperfusion with addition of various concentrations of CuSO4 to the perfused rat livers was tested. The aim of this study was to decide whether the toxicity of copper in liver perfusates is related to protein oxidation and oxidative DNA damage. The high contribution to the DNA damage can be related to the physical liver manipulation during harvest and reperfusion as well as to artefacts induced during the sample preparation (time-consuming sample handling during DNA isolation and extraction). The obtained results pointed out that the DNA damage occurred already during liver handling even before application of CuSO4, whereby concentration of CuSO4 higher than 0.03 mmol L–1 caused a total liver damage, which led to a complete stop of the flow of the perfusate.
650    12
$a měď $x toxicita $7 D003300
650    12
$a nemoci jater $7 D008107
650    12
$a poškození DNA $7 D004249
650    _2
$a oxidační stres $7 D018384
650    _2
$a deoxyguanosin $x chemie $7 D003849
650    _2
$a biologické markery $7 D015415
650    12
$a vysokoúčinná kapalinová chromatografie $x metody $x využití $7 D002851
650    12
$a elektrochemické techniky $x metody $x využití $7 D055664
650    _2
$a spektrofotometrie $x metody $x využití $7 D013053
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a zvířata $7 D000818
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kebis, Anton $7 _AN043859 $u Fakulta verejného zdravotníctva, Slovenská zdravotnícka univerzita v Bratislave
700    1_
$a Príbojová, Jana $7 xx0224794 $u Fakulta ošetrovateľstva a zdravotnických odborných štúdií, Slovenská zdravotnícka univerzita v Bratislave
700    1_
$a Valachovičová, Martina $7 xx0224779 $u Fakulta ošetrovateľstva a zdravotnických odborných štúdií, Slovenská zdravotnícka univerzita v Bratislave
773    0_
$t Chemické listy $x 0009-2770 $g Roč. 110, č. 1 (2016), s. 53-58 $w MED00011010
856    41
$u http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/262/262 $y domovská stránka časopisu
910    __
$a ABA008 $b B 1918 $c 395 $y 4 $z 0
990    __
$a 20160523135344 $b ABA008
991    __
$a 20180530073443 $b ABA008
999    __
$a ok $b bmc $g 1125269 $s 939521
BAS    __
$a 3
BMC    __
$a 2016 $b 110 $c 1 $d 53-58 $i 0009-2770 $m Chemické listy $n Chem. Listy $x MED00011010
LZP    __
$c NLK137 $d 20160627 $a NLK 2016-19/pk

Najít záznam

Citační ukazatele

Možnosti archivace