• Je něco špatně v tomto záznamu ?

A genomic and transcriptomic approach to investigate the blue pigment phenotype in Pseudomonas fluorescens

NA. Andreani, L. Carraro, ME. Martino, M. Fondi, L. Fasolato, G. Miotto, M. Magro, F. Vianello, B. Cardazzo,

. 2015 ; 213 (-) : 88-98. [pub] 20150601

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16020704

Pseudomonas fluorescens is a well-known food spoiler, able to cause serious economic losses in the food industry due to its ability to produce many extracellular, and often thermostable, compounds. The most outstanding spoilage events involving P. fluorescens were blue discoloration of several food stuffs, mainly dairy products. The bacteria involved in such high-profile cases have been identified as belonging to a clearly distinct phylogenetic cluster of the P. fluorescens group. Although the blue pigment has recently been investigated in several studies, the biosynthetic pathway leading to the pigment formation, as well as its chemical nature, remain challenging and unsolved points. In the present paper, genomic and transcriptomic data of 4 P. fluorescens strains (2 blue-pigmenting strains and 2 non-pigmenting strains) were analyzed to evaluate the presence and the expression of blue strain-specific genes. In particular, the pangenome analysis showed the presence in the blue-pigmenting strains of two copies of genes involved in the tryptophan biosynthesis pathway (including trpABCDF). The global expression profiling of blue-pigmenting strains versus non-pigmenting strains showed a general up-regulation of genes involved in iron uptake and a down-regulation of genes involved in primary metabolism. Chromogenic reaction of the blue-pigmenting bacterial cells with Kovac's reagent indicated an indole-derivative as the precursor of the blue pigment. Finally, solubility tests and MALDI-TOF mass spectrometry analysis of the isolated pigment suggested that its molecular structure is very probably a hydrophobic indigo analog.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16020704
003      
CZ-PrNML
005      
20160728105038.0
007      
ta
008      
160722s2015 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.ijfoodmicro.2015.05.024 $2 doi
024    7_
$a 10.1016/j.ijfoodmicro.2015.05.024 $2 doi
035    __
$a (PubMed)26051958
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Andreani, Nadia Andrea $u Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università, 16 Legnaro, 35020 Padova, Italy. $7 gn_A_00006390
245    12
$a A genomic and transcriptomic approach to investigate the blue pigment phenotype in Pseudomonas fluorescens / $c NA. Andreani, L. Carraro, ME. Martino, M. Fondi, L. Fasolato, G. Miotto, M. Magro, F. Vianello, B. Cardazzo,
520    9_
$a Pseudomonas fluorescens is a well-known food spoiler, able to cause serious economic losses in the food industry due to its ability to produce many extracellular, and often thermostable, compounds. The most outstanding spoilage events involving P. fluorescens were blue discoloration of several food stuffs, mainly dairy products. The bacteria involved in such high-profile cases have been identified as belonging to a clearly distinct phylogenetic cluster of the P. fluorescens group. Although the blue pigment has recently been investigated in several studies, the biosynthetic pathway leading to the pigment formation, as well as its chemical nature, remain challenging and unsolved points. In the present paper, genomic and transcriptomic data of 4 P. fluorescens strains (2 blue-pigmenting strains and 2 non-pigmenting strains) were analyzed to evaluate the presence and the expression of blue strain-specific genes. In particular, the pangenome analysis showed the presence in the blue-pigmenting strains of two copies of genes involved in the tryptophan biosynthesis pathway (including trpABCDF). The global expression profiling of blue-pigmenting strains versus non-pigmenting strains showed a general up-regulation of genes involved in iron uptake and a down-regulation of genes involved in primary metabolism. Chromogenic reaction of the blue-pigmenting bacterial cells with Kovac's reagent indicated an indole-derivative as the precursor of the blue pigment. Finally, solubility tests and MALDI-TOF mass spectrometry analysis of the isolated pigment suggested that its molecular structure is very probably a hydrophobic indigo analog.
650    _2
$a mléčné výrobky $x mikrobiologie $7 D003611
650    _2
$a down regulace $7 D015536
650    _2
$a energetický metabolismus $x genetika $7 D004734
650    12
$a potravinářská mikrobiologie $7 D005516
650    _2
$a stanovení celkové genové exprese $7 D020869
650    _2
$a genomika $7 D023281
650    _2
$a oxidoreduktasy $x genetika $7 D010088
650    _2
$a spotřeba kyslíku $x genetika $7 D010101
650    _2
$a fenotyp $7 D010641
650    _2
$a fylogeneze $7 D010802
650    _2
$a biologické pigmenty $x genetika $7 D010860
650    _2
$a Pseudomonas fluorescens $x genetika $x metabolismus $7 D011551
650    _2
$a transkriptom $x genetika $7 D059467
650    _2
$a tryptofan $x biosyntéza $7 D014364
650    _2
$a upregulace $7 D015854
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Carraro, Lisa $u Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università, 16 Legnaro, 35020 Padova, Italy.
700    1_
$a Martino, Maria Elena $u Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università, 16 Legnaro, 35020 Padova, Italy; Institute of Functional Genomics (IGFL), 34-35 Avenue Tony Garnier, 69007 Lyon, France.
700    1_
$a Fondi, Marco $u Florence Computational Biology Group (ComBo), Department of Biology, University of Florence, Via Madonna del Piano, 6 Sesto Fiorentino, 50019 Florence, Italy.
700    1_
$a Fasolato, Luca $u Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università, 16 Legnaro, 35020 Padova, Italy.
700    1_
$a Miotto, Giovanni $u Department of Molecular Medicine, University of Padova, Via Gabelli 63, 35121 Padova, Italy.
700    1_
$a Magro, Massimiliano $u Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università, 16 Legnaro, 35020 Padova, Italy; Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Palacky University, 17. Listopadu 1192/12, 771 46 Olomouc, Czech Republic.
700    1_
$a Vianello, Fabio $u Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università, 16 Legnaro, 35020 Padova, Italy; Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Palacky University, 17. Listopadu 1192/12, 771 46 Olomouc, Czech Republic.
700    1_
$a Cardazzo, Barbara $u Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università, 16 Legnaro, 35020 Padova, Italy. Electronic address: barbara.cardazzo@unipd.it.
773    0_
$w MED00002320 $t International journal of food microbiology $x 1879-3460 $g Roč. 213, č. - (2015), s. 88-98
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26051958 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20160728105300 $b ABA008
999    __
$a ok $b bmc $g 1155374 $s 945232
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 213 $c - $d 88-98 $e 20150601 $i 1879-3460 $m International journal of food microbiology $n Int J Food Microbiol $x MED00002320
LZP    __
$a Pubmed-20160722

Najít záznam

Citační ukazatele

Možnosti archivace