• Something wrong with this record ?

Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression

E Malmstrom, A Davidova, M Morgelin, A Linder, M Larsen, K Qvortrup, P Nordenfelt, O Shannon, O Dzupova, M Holub, J Malmstrom, H Herwald

. 2014 ; 112 (6) : 1230-1243.

Language English Country Germany

Persistent link   https://www.medvik.cz/link/bmc17011780

Grant support
NT11390 MZ0 CEP Register

Early diagnosis of severe infectious diseases is essential for timely implementation of lifesaving therapies. In a search for novel biomarkers in sepsis diagnosis we focused on polymorphonuclear neutrophils (PMNs). Notably, PMNs have their protein cargo readily stored in granules and following systemic stimulation, an immediate increase of neutrophil-borne proteins can be observed into the circulation of sepsis patients. We applied a combination of mass spectrometry (MS) based approaches, LC-MS/MS and selected reaction monitoring (SRM), to characterise and quantify the neutrophil proteome in healthy or disease conditions. With this approach we identified a neutrophil-derived protein abundance pattern in blood plasma consisting of 20 proteins that can be used as a protein signature for severe infectious diseases. Our results also show that SRM is highly sensitive, specific, and reproducible and, thus, a promising technology to study a complex, dynamic and multifactorial disease such as sepsis.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17011780
003      
CZ-PrNML
005      
20170404150218.0
007      
ta
008      
170404s2014 gw d f 000 0|eng||
009      
AR
024    7_
$a 10.1160/TH14-04-0312 $2 doi
035    __
$a (PubMed)25104417
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Malmström, Erik $u Malmstrom, E. Erik Malmstrom, Department of Clinical Sciences, Division of Infection Medicine, , Lund University, Lund, Sweden,
245    10
$a Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression / $c E Malmstrom, A Davidova, M Morgelin, A Linder, M Larsen, K Qvortrup, P Nordenfelt, O Shannon, O Dzupova, M Holub, J Malmstrom, H Herwald
520    9_
$a Early diagnosis of severe infectious diseases is essential for timely implementation of lifesaving therapies. In a search for novel biomarkers in sepsis diagnosis we focused on polymorphonuclear neutrophils (PMNs). Notably, PMNs have their protein cargo readily stored in granules and following systemic stimulation, an immediate increase of neutrophil-borne proteins can be observed into the circulation of sepsis patients. We applied a combination of mass spectrometry (MS) based approaches, LC-MS/MS and selected reaction monitoring (SRM), to characterise and quantify the neutrophil proteome in healthy or disease conditions. With this approach we identified a neutrophil-derived protein abundance pattern in blood plasma consisting of 20 proteins that can be used as a protein signature for severe infectious diseases. Our results also show that SRM is highly sensitive, specific, and reproducible and, thus, a promising technology to study a complex, dynamic and multifactorial disease such as sepsis.
590    __
$a bohemika - dle Pubmed
650    02
$a biologické markery $x metabolismus $7 D015415
650    12
$a krevní proteiny $x metabolismus $7 D001798
650    02
$a studie případů a kontrol $7 D016022
650    12
$a chromatografie kapalinová $7 D002853
650    02
$a progrese nemoci $7 D018450
650    02
$a lidé $7 D006801
650    12
$a neutrofily $x imunologie $x metabolismus $x mikrobiologie $7 D009504
650    02
$a prediktivní hodnota testů $7 D011237
650    12
$a proteomika $x metody $7 D040901
650    12
$a sepse $7 D018805
650    02
$a stupeň závažnosti nemoci $7 D012720
650    12
$a tandemová hmotnostní spektrometrie $7 D053719
700    1_
$a Davidová, Alžběta. $u Department of Infectious and Tropical Diseases, First Faculty of Medicine, Charles University in Prague and Na Bulovce Hospital, Prague Czech Republic; Department of Infectious Diseases, First Faculty of Medicine Military University Hospital and Charles University in Prague, Prague Czech Republic $7 xx0212482
700    1_
$a Mörgelin, Matthias
700    1_
$a Linder, Adam
700    1_
$a Larsen, Michael
700    1_
$a Qvortrup, Klaus
700    1_
$a Nordenfelt, Pontus
700    1_
$a Shannon, Oonagh
700    1_
$a Džupová, Olga $7 xx0080446 $u Department of Infectious, Third Faculty of Medicine, Charles University in Prague and Na Bulovce Hospital, Prague Czech Republic
700    1_
$a Holub, Michal $7 xx0018615 $u Department of Infectious and Tropical Diseases, First Faculty of Medicine, Charles University in Prague and Na Bulovce Hospital, Prague Czech Republic; Department of Infectious Diseases, First Faculty of Medicine Military University Hospital and Charles University in Prague, Prague Czech Republic
700    1_
$a Malmström, Johan
700    1_
$a Herwald, Heiko
773    0_
$t Thrombosis and haemostasis $x 0340-6245 $g Roč. 112, č. 6 (2014), s. 1230-1243 $p Thromb Haemost $w MED00004517
773    0_
$p Thromb Haemost $g 112(6):1230-43, 2014 Dec $x 0340-6245
910    __
$a ABA008 $y 4 $z 0
990    __
$a 20170404150508 $b ABA008
991    __
$a 20170404150508 $b ABA008
999    __
$a ok $b bmc $g 1197372 $s 972539
BAS    __
$a 3
BMC    __
$a 2014 $b 112 $c 6 $d 1230-1243 $x MED00004517 $i 0340-6245 $m Thrombosis and haemostasis
GRA    __
$a NT11390 $p MZ0
LZP    __
$a NLK 2015-B/lp

Find record

Citation metrics

Archiving options