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Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system
P. Marsik, M. Sisa, O. Lacina, K. Motkova, L. Langhansova, J. Rezek, T. Vanek,
Language English Country England, Great Britain
Document type Journal Article
- MeSH
- Anti-Inflammatory Agents, Non-Steroidal metabolism MeSH
- Arabidopsis metabolism MeSH
- Mass Spectrometry MeSH
- Hydroxylation MeSH
- Ibuprofen analysis metabolism MeSH
- Plants metabolism MeSH
- Suspensions MeSH
- Publication type
- Journal Article MeSH
The uptake and metabolism of ibuprofen (IBU) by plants at the cellular level was investigated using a suspension culture of A. thaliana. Almost all IBU added to the medium (200 μM) was metabolized or bound to insoluble structures in 5 days. More than 300 metabolites were determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis, and most of these are first reported for plants here. Although hydroxylated derivatives formed by oxidation on the isobutyl side chain were the main first-step products of IBU degradation, conjugates of these products with sugar, methyl and amino acid groups were the dominant metabolites in the culture. The main portion of total added IBU (81%) was accumulated in the extractable intracellular pool, whereas the cultivation medium fraction contained only 19%. The amount of the insoluble cell-wall-bound IBU was negligible (0.005% of total IBU).
HPST s r o Písnická 372 20 142 00 Prague Czech Republic
Institute of Experimental Botany AS CR Rozvojova 263 165 02 Prague Czech Republic
References provided by Crossref.org
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- $a The uptake and metabolism of ibuprofen (IBU) by plants at the cellular level was investigated using a suspension culture of A. thaliana. Almost all IBU added to the medium (200 μM) was metabolized or bound to insoluble structures in 5 days. More than 300 metabolites were determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis, and most of these are first reported for plants here. Although hydroxylated derivatives formed by oxidation on the isobutyl side chain were the main first-step products of IBU degradation, conjugates of these products with sugar, methyl and amino acid groups were the dominant metabolites in the culture. The main portion of total added IBU (81%) was accumulated in the extractable intracellular pool, whereas the cultivation medium fraction contained only 19%. The amount of the insoluble cell-wall-bound IBU was negligible (0.005% of total IBU).
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