-
Je něco špatně v tomto záznamu ?
Nerolidol and Farnesol Inhibit Some Cytochrome P450 Activities but Did Not Affect Other Xenobiotic-Metabolizing Enzymes in Rat and Human Hepatic Subcellular Fractions
A. Špičáková, B. Szotáková, D. Dimunová, Z. Myslivečková, V. Kubíček, M. Ambrož, K. Lněničková, K. Krasulová, P. Anzenbacher, L. Skálová,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 1997
Free Medical Journals
od 1997
PubMed Central
od 2001
Europe PubMed Central
od 2001
ProQuest Central
od 1997-01-01
Open Access Digital Library
od 1997-01-01
Medline Complete (EBSCOhost)
od 2009-03-01
Health & Medicine (ProQuest)
od 1997-01-01
- MeSH
- farnesol chemie farmakologie MeSH
- inhibiční koncentrace 50 MeSH
- inhibitory cytochromu P450 chemie farmakologie MeSH
- játra enzymologie MeSH
- kinetika MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- seskviterpeny chemie farmakologie MeSH
- subcelulární frakce enzymologie MeSH
- systém (enzymů) cytochromů P-450 metabolismus MeSH
- xenobiotika metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Sesquiterpenes, 15-carbon compounds formed from three isoprenoid units, are the main components of plant essential oils. Sesquiterpenes occur in human food, but they are principally taken as components of many folk medicines and dietary supplements. The aim of our study was to test and compare the potential inhibitory effect of acyclic sesquiterpenes, trans-nerolidol, cis-nerolidol and farnesol, on the activities of the main xenobiotic-metabolizing enzymes in rat and human liver in vitro. Rat and human subcellular fractions, relatively specific substrates, corresponding coenzymes and HPLC, spectrophotometric or spectrofluorometric analysis of product formation were used. The results showed significant inhibition of cytochromes P450 (namely CYP1A, CYP2B and CYP3A subfamilies) activities by all tested sesquiterpenes in rat as well as in human hepatic microsomes. On the other hand, all tested sesquiterpenes did not significantly affect the activities of carbonyl-reducing enzymes and conjugation enzymes. The results indicate that acyclic sesquiterpenes might affect CYP1A, CYP2B and CYP3A mediated metabolism of concurrently administered drugs and other xenobiotics. The possible drug-sesquiterpene interactions should be verified in in vivo experiments.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17023180
- 003
- CZ-PrNML
- 005
- 20180214201948.0
- 007
- ta
- 008
- 170720s2017 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.3390/molecules22040509 $2 doi
- 035 __
- $a (PubMed)28338641
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Špičáková, Alena $u Department of Pharmacology and Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacky University, Hněvotínská 3, 77515 Olomouc, Czech Republic. alena.spicakova@upol.cz.
- 245 10
- $a Nerolidol and Farnesol Inhibit Some Cytochrome P450 Activities but Did Not Affect Other Xenobiotic-Metabolizing Enzymes in Rat and Human Hepatic Subcellular Fractions / $c A. Špičáková, B. Szotáková, D. Dimunová, Z. Myslivečková, V. Kubíček, M. Ambrož, K. Lněničková, K. Krasulová, P. Anzenbacher, L. Skálová,
- 520 9_
- $a Sesquiterpenes, 15-carbon compounds formed from three isoprenoid units, are the main components of plant essential oils. Sesquiterpenes occur in human food, but they are principally taken as components of many folk medicines and dietary supplements. The aim of our study was to test and compare the potential inhibitory effect of acyclic sesquiterpenes, trans-nerolidol, cis-nerolidol and farnesol, on the activities of the main xenobiotic-metabolizing enzymes in rat and human liver in vitro. Rat and human subcellular fractions, relatively specific substrates, corresponding coenzymes and HPLC, spectrophotometric or spectrofluorometric analysis of product formation were used. The results showed significant inhibition of cytochromes P450 (namely CYP1A, CYP2B and CYP3A subfamilies) activities by all tested sesquiterpenes in rat as well as in human hepatic microsomes. On the other hand, all tested sesquiterpenes did not significantly affect the activities of carbonyl-reducing enzymes and conjugation enzymes. The results indicate that acyclic sesquiterpenes might affect CYP1A, CYP2B and CYP3A mediated metabolism of concurrently administered drugs and other xenobiotics. The possible drug-sesquiterpene interactions should be verified in in vivo experiments.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a inhibitory cytochromu P450 $x chemie $x farmakologie $7 D065607
- 650 _2
- $a systém (enzymů) cytochromů P-450 $x metabolismus $7 D003577
- 650 _2
- $a farnesol $x chemie $x farmakologie $7 D005204
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a inhibiční koncentrace 50 $7 D020128
- 650 _2
- $a kinetika $7 D007700
- 650 _2
- $a játra $x enzymologie $7 D008099
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a seskviterpeny $x chemie $x farmakologie $7 D012717
- 650 _2
- $a subcelulární frakce $x enzymologie $7 D013347
- 650 _2
- $a xenobiotika $x metabolismus $7 D015262
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Szotáková, Barbora $u Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic. szotakova@faf.cuni.cz.
- 700 1_
- $a Dimunová, Diana $u Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic. kristyna.krasulova@upol.cz.
- 700 1_
- $a Myslivečková, Zuzana $u Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic. pavel.anzenbacher@upol.cz.
- 700 1_
- $a Kubíček, Vladimír $u Department of Biophysics and Physical Chemistry, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic. dimunovd@faf.cuni.cz.
- 700 1_
- $a Ambrož, Martin $u Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic. zuzka.mysliveckova@seznam.cz. $7 gn_A_00005430
- 700 1_
- $a Lněničková, Kateřina $u Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic. ambrozm@faf.cuni.cz.
- 700 1_
- $a Krasulová, Kristýna $u Department of Pharmacology and Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacky University, Hněvotínská 3, 77515 Olomouc, Czech Republic. lnenickk@faf.cuni.cz.
- 700 1_
- $a Anzenbacher, Pavel $u Department of Pharmacology and Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacky University, Hněvotínská 3, 77515 Olomouc, Czech Republic. skaloval@faf.cuni.cz. $7 xx0034447 $4
- 700 1_
- $a Skálová, Lenka $u Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic. kubicek@faf.cuni.cz.
- 773 0_
- $w MED00180394 $t Molecules (Basel, Switzerland) $x 1420-3049 $g Roč. 22, č. 4 (2017)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28338641 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20170720 $b ABA008
- 991 __
- $a 20170720124639 $b ABA008
- 999 __
- $a ok $b bmc $g 1238861 $s 984093
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 22 $c 4 $e 20170324 $i 1420-3049 $m Molecules $n Molecules $x MED00180394
- LZP __
- $a Pubmed-20170720
