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MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

H. Uhlíková, M. Solanský, V. Hrdinová, O. Šedo, T. Kašparovský, J. Hejátko, J. Lochman,

. 2017 ; 210 (-) : 51-57. [pub] 20161214

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17023385

Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins.

Citace poskytuje Crossref.org

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$a Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins.
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$a Hrdinová, Vendula $u Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czechia; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia.
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$a Šedo, Ondrej $u Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czechia; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia.
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$a Lochman, Jan $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czechia. Electronic address: lochik@mail.muni.cz.
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