Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Gelatinases A and B and antioxidant enzyme activity in the early phase of acute myocardial infarction

K. Gopcevic, B. Rovcanin, D. Kekic, D. Milasinovic, G. Kocic, I. Stojanovic

. 2017 ; 63 (1) : 20-26.

Language English Country Czech Republic

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17024929

Oxidative stress plays important roles in the pathophysiology of acute myocardial infarction (AMI). The aim of this study was to investigate the correlation of the oxidative stress status and matrix metalloproteinase activity in AMI patients in comparison to controls. This study included 136 subjects: 68 patients with AMI (42 males/26 females; mean age 58.5 ± 10.5 years) and 68 controls (37 males/29 females; mean age 60.2 ± 12.4 years). Gelatinases A and B were assayed using gelatin zymography, enzyme activities were obtained spectrophotometrically. Gelatinase A and B activities were increased in the AMI patients' group compared to the control. Activities of serum superoxide dismutase (SOD) and xanthine oxidase (XO) were significantly higher in AMI patients (106.53 ± 23.45 U/l, P < 0.001 and 158.18 ± 29.59 U/l, P < 0.001) than in the control group (55.99 ± 10.79 U/l and 79.81 ± 7.93 U/l). The activity of catalase (CAT) in the sera of AMI patients was lower (271.31 ± 7.53 U/l, P < 0.005) than in the control group (305.94 ± 97.28 U/l). Plasma glutathione peroxidase (GPx) and glutathione reductase (GR) in AMI patients were significantly higher (582.47 ± 184.81 U/l, P < 0.001 and 59.64 ± 21.88 U/l, P < 0.001) than in the control group (275.32 ± 104.69 U/l and 47.71 ± 20.05 U/l). The present findings demonstrate activation of gelatinases A and B and oxidative stress markers in the early stage of AMI. Gelatinases, detected at high levels in AMI patients only, indicate their noticeable predisposition for becoming additional biomarkers of the early phase of AMI.

000      
00000naa a2200000 a 4500
001      
bmc17024929
003      
CZ-PrNML
005      
20211129144444.0
007      
ta
008      
170804s2017 xr d f 000 0|eng||
009      
AR
035    __
$a (PubMed)28374671
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Gopcevic, K. $u Institute for Chemistry in Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
245    10
$a Gelatinases A and B and antioxidant enzyme activity in the early phase of acute myocardial infarction / $c K. Gopcevic, B. Rovcanin, D. Kekic, D. Milasinovic, G. Kocic, I. Stojanovic
520    9_
$a Oxidative stress plays important roles in the pathophysiology of acute myocardial infarction (AMI). The aim of this study was to investigate the correlation of the oxidative stress status and matrix metalloproteinase activity in AMI patients in comparison to controls. This study included 136 subjects: 68 patients with AMI (42 males/26 females; mean age 58.5 ± 10.5 years) and 68 controls (37 males/29 females; mean age 60.2 ± 12.4 years). Gelatinases A and B were assayed using gelatin zymography, enzyme activities were obtained spectrophotometrically. Gelatinase A and B activities were increased in the AMI patients' group compared to the control. Activities of serum superoxide dismutase (SOD) and xanthine oxidase (XO) were significantly higher in AMI patients (106.53 ± 23.45 U/l, P < 0.001 and 158.18 ± 29.59 U/l, P < 0.001) than in the control group (55.99 ± 10.79 U/l and 79.81 ± 7.93 U/l). The activity of catalase (CAT) in the sera of AMI patients was lower (271.31 ± 7.53 U/l, P < 0.005) than in the control group (305.94 ± 97.28 U/l). Plasma glutathione peroxidase (GPx) and glutathione reductase (GR) in AMI patients were significantly higher (582.47 ± 184.81 U/l, P < 0.001 and 59.64 ± 21.88 U/l, P < 0.001) than in the control group (275.32 ± 104.69 U/l and 47.71 ± 20.05 U/l). The present findings demonstrate activation of gelatinases A and B and oxidative stress markers in the early stage of AMI. Gelatinases, detected at high levels in AMI patients only, indicate their noticeable predisposition for becoming additional biomarkers of the early phase of AMI.
650    _2
$a senioři $7 D000368
650    _2
$a antioxidancia $x metabolismus $7 D000975
650    _2
$a studie případů a kontrol $7 D016022
650    _2
$a katalasa $x krev $7 D002374
650    _2
$a želatinasy $x krev $x metabolismus $7 D018093
650    _2
$a glutathionperoxidasa $x krev $7 D005979
650    _2
$a glutathionreduktasa $x krev $7 D005980
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a infarkt myokardu $x krev $x enzymologie $7 D009203
650    _2
$a dusičnany $x krev $7 D009566
650    _2
$a dusitany $x krev $7 D009573
650    _2
$a protein - isoformy $x krev $7 D020033
650    _2
$a referenční standardy $7 D012015
650    _2
$a superoxiddismutasa $7 D013482
650    _2
$a xanthinoxidasa $x krev $7 D014969
655    _2
$a časopisecké články $7 D016428
700    1_
$a Rovcanin, B. $u Centre for Endocrine Surgery, Clinical Centre of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
700    1_
$a Kekic, D. $u Institute for Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
700    1_
$a Milasinovic, D. $u Clinic for Cardiology, Clinical Centre of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
700    1_
$a Kocic, G. $u Institute for Biochemistry, Faculty of Medicine, University of Nis, Nis, Serbia
700    1_
$a Stojanovic, I. $u Institute for Biochemistry, Faculty of Medicine, University of Nis, Nis, Serbia
773    0_
$w MED00011004 $t Folia biologica $x 0015-5500 $g Roč. 63, č. 1 (2017), s. 20-26
856    41
$u https://fb.cuni.cz/file/5833/fb2017a0004.pdf $y plný text volně přístupný
910    __
$a ABA008 $b A 970 $c 89 $y 4 $z 0
990    __
$a 20170804 $b ABA008
991    __
$a 20211129144443 $b ABA008
999    __
$a ok $b bmc $g 1245304 $s 985848
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 63 $c 1 $d 20-26 $i 0015-5500 $m Folia biologica (Praha) $n Folia biol. (Praha) $x MED00011004
LZP    __
$b NLK118 $a Pubmed-20170804

Find record

Citation metrics

Archiving options