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Effects of selected Indonesian plant extracts on E. cuniculi infection in vivo
B. Sak, A. Jandová, K. Doležal, M. Kváč, D. Květoňová, L. Hlásková, M. Rost, M. Olšanský, W. Nurcahyo, I. Foitová,
Language English Country United States
Document type Journal Article
- MeSH
- Albendazole pharmacology therapeutic use MeSH
- Antifungal Agents pharmacology therapeutic use MeSH
- Chlorocebus aethiops MeSH
- Dimethyl Sulfoxide pharmacology therapeutic use MeSH
- Diospyros chemistry MeSH
- Dipterocarpaceae chemistry MeSH
- DNA, Fungal isolation & purification MeSH
- Encephalitozoon cuniculi drug effects MeSH
- Encephalitozoonosis drug therapy MeSH
- Fabaceae chemistry MeSH
- Feces parasitology MeSH
- Gastrointestinal Tract microbiology MeSH
- Immunocompetence MeSH
- Plant Leaves chemistry MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Piper betle chemistry MeSH
- Plant Extracts pharmacology therapeutic use MeSH
- Spores, Fungal drug effects MeSH
- Vero Cells MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Indonesia MeSH
The present study was conducted to evaluate the methanolic extracts from several plant leaves widely used in traditional medicine to cure digestive tract disorders and in the self-medication of wild animals such as non-human primates, namely Archidendron fagifolium, Diospyros sumatrana, Shorea sumatrana, and Piper betle leaves, with regard to their antimicrosporidial activity against Encephalitozoon cuniculi in immunocompetent BALB/c mice determined using molecular detection of microsporidial DNA (qPCR) in various tissues and body fluids of infected, treated mice. Of the plant extracts tested, Diospyros sumatrana provided the most promising results, reducing spore shedding by 88% compared to untreated controls. Moreover, total burden per 1 g of tissue in the D. sumatrana extract-treated group reached 87% reduction compared to untreated controls, which was comparable to the effect of the standard drug, Albendazole. This data represents the baseline necessary for further research focused on determining the structure, activity and modes of action of the active compounds, mainly of D. sumatrana, enabling subsequent development of antimicrosporidial remedies.
Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic
Faculty of Economics University of South Bohemia in České Budějovice České Budějovice Czech Republic
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
References provided by Crossref.org
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- $a The present study was conducted to evaluate the methanolic extracts from several plant leaves widely used in traditional medicine to cure digestive tract disorders and in the self-medication of wild animals such as non-human primates, namely Archidendron fagifolium, Diospyros sumatrana, Shorea sumatrana, and Piper betle leaves, with regard to their antimicrosporidial activity against Encephalitozoon cuniculi in immunocompetent BALB/c mice determined using molecular detection of microsporidial DNA (qPCR) in various tissues and body fluids of infected, treated mice. Of the plant extracts tested, Diospyros sumatrana provided the most promising results, reducing spore shedding by 88% compared to untreated controls. Moreover, total burden per 1 g of tissue in the D. sumatrana extract-treated group reached 87% reduction compared to untreated controls, which was comparable to the effect of the standard drug, Albendazole. This data represents the baseline necessary for further research focused on determining the structure, activity and modes of action of the active compounds, mainly of D. sumatrana, enabling subsequent development of antimicrosporidial remedies.
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