The present study was conducted to evaluate the methanolic extracts from several plant leaves widely used in traditional medicine to cure digestive tract disorders and in the self-medication of wild animals such as non-human primates, namely Archidendron fagifolium, Diospyros sumatrana, Shorea sumatrana, and Piper betle leaves, with regard to their antimicrosporidial activity against Encephalitozoon cuniculi in immunocompetent BALB/c mice determined using molecular detection of microsporidial DNA (qPCR) in various tissues and body fluids of infected, treated mice. Of the plant extracts tested, Diospyros sumatrana provided the most promising results, reducing spore shedding by 88% compared to untreated controls. Moreover, total burden per 1 g of tissue in the D. sumatrana extract-treated group reached 87% reduction compared to untreated controls, which was comparable to the effect of the standard drug, Albendazole. This data represents the baseline necessary for further research focused on determining the structure, activity and modes of action of the active compounds, mainly of D. sumatrana, enabling subsequent development of antimicrosporidial remedies.

Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic

Department of Chemical Biology and Genetics Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Olomouc Czech Republic

Department of Parasitology Faculty of Veterinary Medicine Gadjah Mada University Yogyakarta Indonesia

Faculty of Agriculture University of South Bohemia in České Budějovice České Budějovice Czech Republic

Faculty of Economics University of South Bohemia in České Budějovice České Budějovice Czech Republic

Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic

Laboratory of Growth Regulators Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacky University and Institute of Experimental Botany Czech Academy of Sciences Olomouc Czech Republic

UMI Saving of Pongidae Foundation Brno Czech Republic

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$a Sak, Bohumil $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic. Electronic address: casio@paru.cas.cz.

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$a Effects of selected Indonesian plant extracts on E. cuniculi infection in vivo / $c B. Sak, A. Jandová, K. Doležal, M. Kváč, D. Květoňová, L. Hlásková, M. Rost, M. Olšanský, W. Nurcahyo, I. Foitová,

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$a The present study was conducted to evaluate the methanolic extracts from several plant leaves widely used in traditional medicine to cure digestive tract disorders and in the self-medication of wild animals such as non-human primates, namely Archidendron fagifolium, Diospyros sumatrana, Shorea sumatrana, and Piper betle leaves, with regard to their antimicrosporidial activity against Encephalitozoon cuniculi in immunocompetent BALB/c mice determined using molecular detection of microsporidial DNA (qPCR) in various tissues and body fluids of infected, treated mice. Of the plant extracts tested, Diospyros sumatrana provided the most promising results, reducing spore shedding by 88% compared to untreated controls. Moreover, total burden per 1 g of tissue in the D. sumatrana extract-treated group reached 87% reduction compared to untreated controls, which was comparable to the effect of the standard drug, Albendazole. This data represents the baseline necessary for further research focused on determining the structure, activity and modes of action of the active compounds, mainly of D. sumatrana, enabling subsequent development of antimicrosporidial remedies.

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$a Jandová, Anna $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.

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$a Doležal, Karel $u Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic; Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University & Institute of Experimental Botany, Czech Academy of Sciences, Olomouc, Czech Republic.

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$a Kváč, Martin $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.

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$a Květoňová, Dana $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.

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$a Hlásková, Lenka $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.

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$a Rost, Michael $u Faculty of Economics, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.

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$a Olšanský, Milan $u UMI - Saving of Pongidae Foundation, Brno, Czech Republic.

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$a Nurcahyo, Wisnu $u Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.

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$a Foitová, Ivona $u Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia; Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic.

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