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Moraxella catarrhalis Might Be More Common than Expected in Acute Otitis Media in Young Finnish Children
S. Sillanpää, S. Oikarinen, M. Sipilä, L. Kramna, M. Rautiainen, H. Huhtala, J. Aittoniemi, J. Laranne, H. Hyöty, O. Cinek,
Language English Country United States
Document type Journal Article
NLK
Free Medical Journals
from 1975 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1975 to 1 year ago
Europe PubMed Central
from 1975 to 6 months ago
Open Access Digital Library
from 1975-01-01
Open Access Digital Library
from 1975-01-01
PubMed
27413187
DOI
10.1128/jcm.01146-16
Knihovny.cz E-resources
- MeSH
- Bacteriological Techniques methods MeSH
- Gram-Negative Bacterial Infections epidemiology microbiology MeSH
- Gram-Positive Bacterial Infections epidemiology microbiology MeSH
- Coinfection epidemiology microbiology MeSH
- Infant MeSH
- Real-Time Polymerase Chain Reaction methods MeSH
- Humans MeSH
- Moraxella catarrhalis isolation & purification MeSH
- Otitis Media epidemiology microbiology MeSH
- Child, Preschool MeSH
- Prevalence MeSH
- Check Tag
- Infant MeSH
- Humans MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Finland epidemiology MeSH
According to studies based on bacterial cultures of middle ear fluids, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis have been the most common pathogens in acute otitis media. However, bacterial culture can be affected by reduced viability or suboptimal growth of bacteria. PCR detects bacterial DNA from samples with greater sensitivity than culture. In the present study, we analyzed the middle ear pathogens with both conventional culture and semiquantitative real-time PCR in 90 middle ear fluid samples obtained from children aged 5 to 42 months during acute otitis media episodes. Samples were tested for the presence of S. pneumoniae, H. influenzae, M. catarrhalis, Alloiococcus otitidis, Staphylococcus aureus, and Pseudomonas aeruginosa One or more bacterial pathogens were detected in 42 (47%) samples with culture and in 69 (77%) samples with PCR. According to PCR analysis, M. catarrhalis results were positive in 42 (47%) samples, H. influenzae in 30 (33%), S. pneumoniae in 27 (30%), A. otitidis in 6 (6.7%), S. aureus in 5 (5.6%), and P. aeruginosa in 1 (1.1%). Multibacterial etiology was seen in 34 (38%) samples, and M. catarrhalis was detected in most (85%) of those cases. Fifteen signals for M. catarrhalis were strong, suggesting a highly probable etiological role of the pathogen. In conclusion, even though M. catarrhalis is often a part of mixed flora in acute otitis media, a considerable proportion of cases may be primarily attributable to this pathogen.
Department of Virology School of Medicine University of Tampere Tampere Finland
Fimlab Laboratories Tampere Finland
School of Health Sciences University of Tampere Tampere Finland
References provided by Crossref.org
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- $a Sillanpää, Saara $u Department of Anatomy, School of Medicine, University of Tampere, Tampere, Finland Department of Otorhinolaryngology and Head and Neck Surgery, Tampere University Hospital, and School of Medicine, University of Tampere, Tampere, Finland saara.sillanpaa@uta.fi.
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- $a According to studies based on bacterial cultures of middle ear fluids, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis have been the most common pathogens in acute otitis media. However, bacterial culture can be affected by reduced viability or suboptimal growth of bacteria. PCR detects bacterial DNA from samples with greater sensitivity than culture. In the present study, we analyzed the middle ear pathogens with both conventional culture and semiquantitative real-time PCR in 90 middle ear fluid samples obtained from children aged 5 to 42 months during acute otitis media episodes. Samples were tested for the presence of S. pneumoniae, H. influenzae, M. catarrhalis, Alloiococcus otitidis, Staphylococcus aureus, and Pseudomonas aeruginosa One or more bacterial pathogens were detected in 42 (47%) samples with culture and in 69 (77%) samples with PCR. According to PCR analysis, M. catarrhalis results were positive in 42 (47%) samples, H. influenzae in 30 (33%), S. pneumoniae in 27 (30%), A. otitidis in 6 (6.7%), S. aureus in 5 (5.6%), and P. aeruginosa in 1 (1.1%). Multibacterial etiology was seen in 34 (38%) samples, and M. catarrhalis was detected in most (85%) of those cases. Fifteen signals for M. catarrhalis were strong, suggesting a highly probable etiological role of the pathogen. In conclusion, even though M. catarrhalis is often a part of mixed flora in acute otitis media, a considerable proportion of cases may be primarily attributable to this pathogen.
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