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In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores
M. Petrik, C. Zhai, Z. Novy, L. Urbanek, H. Haas, C. Decristoforo,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu srovnávací studie, časopisecké články
NLK
ProQuest Central
od 2005-01-01 do 2019-01-31
Medline Complete (EBSCOhost)
od 2011-02-01 do Před 1 rokem
Nursing & Allied Health Database (ProQuest)
od 2005-01-01 do 2019-01-31
Health & Medicine (ProQuest)
od 2005-01-01 do 2019-01-31
- MeSH
- myši inbrední BALB C MeSH
- počítačová rentgenová tomografie MeSH
- pozitronová emisní tomografie MeSH
- radioizotopy galia chemie MeSH
- radionuklidy MeSH
- siderofory chemie MeSH
- tkáňová distribuce MeSH
- zirkonium chemie MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
PURPOSE: Some [(68)Ga]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores. PROCEDURES: Radiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice. RESULTS: Certain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [(89)Zr]triacetylfusarinine C (TAFC). All studied Ga-68 and [(89)Zr]siderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of [(89)Zr]ferrioxamine E (FOXE). CONCLUSIONS: Zr-89 and [(68)Ga]siderophores showed analogous in vitro and in vivo behaviour. Tested [(89)Zr]siderophores could be applied for longitudinal positron emission tomography (PET) studies of fungal infections and especially TAFC for the development of novel bioconjugates.
Clinical Department of Nuclear Medicine Medical University Innsbruck Innsbruck Austria
Division of Molecular Biology Biocenter Medical University Innsbruck Innsbruck Austria
Citace poskytuje Crossref.org
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- $a PURPOSE: Some [(68)Ga]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores. PROCEDURES: Radiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice. RESULTS: Certain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [(89)Zr]triacetylfusarinine C (TAFC). All studied Ga-68 and [(89)Zr]siderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of [(89)Zr]ferrioxamine E (FOXE). CONCLUSIONS: Zr-89 and [(68)Ga]siderophores showed analogous in vitro and in vivo behaviour. Tested [(89)Zr]siderophores could be applied for longitudinal positron emission tomography (PET) studies of fungal infections and especially TAFC for the development of novel bioconjugates.
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