• Something wrong with this record ?

A more sensitive detection of micrometastases of NSCLC in lymph nodes using the one-step nucleic acid amplification (OSNA) method

J. Vodicka, P. Mukensnabl, S. Vejvodova, V. Spidlen, V. Kulda, O. Topolcan, M. Pesta,

. 2018 ; 117 (2) : 163-170. [pub] 20171204

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc18010256

BACKGROUND: Detection of tumor cells in lymph nodes (LNs) removed during the treatment of pulmonary tumor by radical surgery is limited by the possibilities of standard histopathological methods. The goal of this study was to obtain more accurate pTNM status by a more sensitive detection of micrometastases in LNs. METHODS: A total of 885 LNs, an average of 13.8 LNs per patient, were removed during 64 surgeries. LNs from the same zone were pooled together as a group, five groups of LNs were examined in each patient. A total of 320 groups of LNs were examined. One-step nucleic acid amplification (OSNA) method was compared to standard histopathological examination with haematoxylin-eosin (H&E) staining and CK19 immunohistochemistry, specifically by an ultimate analysis of all intraoperatively removed LNs. RESULTS: Identical results for H&E and OSNA examinations were recorded in 286 groups of LNs (89.4%). In total, positive examinations were recorded in 27 groups of LNs (8.4%) using the OSNA method, which were H&E negative. In seven groups of LNs (2.2%), the H&E examination was positive, while OSNA method produced negative results. CONCLUSIONS: The OSNA examination led to a higher pTNM stage classification in 14 (21.9%) patients. The clinical significance remains the subject of follow-up research.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18010256
003      
CZ-PrNML
005      
20180416140803.0
007      
ta
008      
180404s2018 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1002/jso.24826 $2 doi
035    __
$a (PubMed)29205350
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Vodicka, Josef $u Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic.
245    12
$a A more sensitive detection of micrometastases of NSCLC in lymph nodes using the one-step nucleic acid amplification (OSNA) method / $c J. Vodicka, P. Mukensnabl, S. Vejvodova, V. Spidlen, V. Kulda, O. Topolcan, M. Pesta,
520    9_
$a BACKGROUND: Detection of tumor cells in lymph nodes (LNs) removed during the treatment of pulmonary tumor by radical surgery is limited by the possibilities of standard histopathological methods. The goal of this study was to obtain more accurate pTNM status by a more sensitive detection of micrometastases in LNs. METHODS: A total of 885 LNs, an average of 13.8 LNs per patient, were removed during 64 surgeries. LNs from the same zone were pooled together as a group, five groups of LNs were examined in each patient. A total of 320 groups of LNs were examined. One-step nucleic acid amplification (OSNA) method was compared to standard histopathological examination with haematoxylin-eosin (H&E) staining and CK19 immunohistochemistry, specifically by an ultimate analysis of all intraoperatively removed LNs. RESULTS: Identical results for H&E and OSNA examinations were recorded in 286 groups of LNs (89.4%). In total, positive examinations were recorded in 27 groups of LNs (8.4%) using the OSNA method, which were H&E negative. In seven groups of LNs (2.2%), the H&E examination was positive, while OSNA method produced negative results. CONCLUSIONS: The OSNA examination led to a higher pTNM stage classification in 14 (21.9%) patients. The clinical significance remains the subject of follow-up research.
650    _2
$a adenokarcinom $x genetika $x sekundární $x chirurgie $7 D000230
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a nádorové biomarkery $x genetika $7 D014408
650    _2
$a nemalobuněčný karcinom plic $x genetika $x sekundární $x chirurgie $7 D002289
650    _2
$a spinocelulární karcinom $x genetika $x sekundární $x chirurgie $7 D002294
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a následné studie $7 D005500
650    _2
$a lidé $7 D006801
650    _2
$a nádory plic $x genetika $x patologie $x chirurgie $7 D008175
650    _2
$a lymfatické uzliny $x metabolismus $x patologie $x chirurgie $7 D008198
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a mikrometastázy $7 D061206
650    _2
$a techniky amplifikace nukleových kyselin $x metody $7 D021141
650    _2
$a prognóza $7 D011379
650    _2
$a prospektivní studie $7 D011446
650    _2
$a messenger RNA $7 D012333
655    _2
$a časopisecké články $7 D016428
700    1_
$a Mukensnabl, Petr $u Sikl's Department of Pathology, University Hospital in Pilsen, Pilsen, Czech Republic.
700    1_
$a Vejvodova, Sarka $u Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic.
700    1_
$a Spidlen, Vladimir $u Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic.
700    1_
$a Kulda, Vlastimil $u Department of Medical Chemistry and Biochemistry, Charles University, Pilsen, Czech Republic.
700    1_
$a Topolcan, Ondrej $u Department of Nuclear Medicine-Immunoanalytic Laboratory, University Hospital in Pilsen, Pilsen, Czech Republic.
700    1_
$a Pesta, Martin $u Department of Biology, Charles University, Pilsen, Czech Republic. Biomedical Centre, Charles University, Pilsen, Czech Republic.
773    0_
$w MED00010662 $t Journal of surgical oncology $x 1096-9098 $g Roč. 117, č. 2 (2018), s. 163-170
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29205350 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180404 $b ABA008
991    __
$a 20180416140900 $b ABA008
999    __
$a ok $b bmc $g 1287741 $s 1007068
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 117 $c 2 $d 163-170 $e 20171204 $i 1096-9098 $m Journal of surgical oncology $n J Surg Oncol $x MED00010662
LZP    __
$a Pubmed-20180404

Find record

Citation metrics

Archiving options