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A porcine model of skin wound infected with a polybacterial biofilm

P. Klein, M. Sojka, J. Kucera, J. Matonohova, V. Pavlik, J. Nemec, G. Kubickova, R. Slavkovsky, K. Szuszkiewicz, P. Danek, M. Rozkot, V. Velebny,

. 2018 ; 34 (2) : 226-236.

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc18024375

A clinically relevant porcine model of a biofilm-infected wound was established in 10 minipigs. The wounds of six experimental animals were infected with a modified polymicrobial Lubbock chronic wound biofilm consisting of Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Bacillus subtilis. Four animals served as uninfected controls. The wounds were monitored until they had healed for 24 days. The biofilm persisted in the wounds up to day 14 and significantly affected healing. The control to infected healed wound area ratios were: 45%/21%, 66%/37%, and 90%/57% on days 7, 10 and 14, respectively. The implanted biofilm prolonged inflammation, increased necrosis, delayed granulation and impaired development of the extracellular matrix as seen in histological and gene expression analyses. This model provides a therapeutic one-week window for testing of anti-biofilm treatments and for research on the pathogenesis of wound infections in pig that is clinically the most relevant animal wound healing model.

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$a A porcine model of skin wound infected with a polybacterial biofilm / $c P. Klein, M. Sojka, J. Kucera, J. Matonohova, V. Pavlik, J. Nemec, G. Kubickova, R. Slavkovsky, K. Szuszkiewicz, P. Danek, M. Rozkot, V. Velebny,
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$a A clinically relevant porcine model of a biofilm-infected wound was established in 10 minipigs. The wounds of six experimental animals were infected with a modified polymicrobial Lubbock chronic wound biofilm consisting of Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Bacillus subtilis. Four animals served as uninfected controls. The wounds were monitored until they had healed for 24 days. The biofilm persisted in the wounds up to day 14 and significantly affected healing. The control to infected healed wound area ratios were: 45%/21%, 66%/37%, and 90%/57% on days 7, 10 and 14, respectively. The implanted biofilm prolonged inflammation, increased necrosis, delayed granulation and impaired development of the extracellular matrix as seen in histological and gene expression analyses. This model provides a therapeutic one-week window for testing of anti-biofilm treatments and for research on the pathogenesis of wound infections in pig that is clinically the most relevant animal wound healing model.
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$a Sojka, Martin $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic. c Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
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$a Kucera, Jan $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic. d Institute of Histology and Embryology, Faculty of Medicine in Hradec Kralove , Charles University , Hradec Kralove , Czech Republic. e Czech Centre for Phenogenomics , Institute of Molecular Genetics/BIOCEV , Vestec , Czech Republic.
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$a Matonohova, Jana $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic.
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$a Pavlik, Vojtech $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic. f Department of Dermatology, Third Faculty of Medicine , Charles University , Prague , Czech Republic.
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$a Nemec, Jan $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic.
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$a Kubickova, Gabriela $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic.
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$a Slavkovsky, Rastislav $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic. g Institute of Molecular and Translational Medicine , Palacky University , Olomouc , Czech Republic.
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$a Szuszkiewicz, Katarzyna $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic. h Department of Analytical Chemistry, Faculty of Chemical Technology , University of Pardubice , Pardubice , Czech Republic.
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$a Danek, Petr $u i Department of Pig Breeding , Institute of Animal Science , Kostelec nad Orlici , Czech Republic.
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$a Rozkot, Miroslav $u i Department of Pig Breeding , Institute of Animal Science , Kostelec nad Orlici , Czech Republic.
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$a Velebny, Vladimir $u b R&D Department , Contipro, a.s. , Dolni Dobrouc , Czech Republic.
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