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Impact of fliD and virulence plasmid pSEV on response of chicken embryo fibroblasts to Salmonella Enteritidis
A. Szmolka, ME. Matulova, I. Rychlik,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- bakteriální proteiny genetika MeSH
- fibroblasty mikrobiologie MeSH
- flagella genetika MeSH
- interakce hostitele a patogenu MeSH
- kuřecí embryo MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- nemoci drůbeže imunologie mikrobiologie MeSH
- plazmidy genetika MeSH
- Salmonella enteritidis genetika patogenita MeSH
- salmonelová infekce u zvířat imunologie mikrobiologie MeSH
- virulence genetika MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Salmonella Enteritidis is the main serovar of poultry origin in humans, but its complex interaction with certain avian cells is still not fully understood. Previously we identified several genes significantly induced in chicken embryo fibroblasts (CEFs) by the wild-type strain S. Enteritidis 11 (SE 11). In the present study, we raised the question whether virulence-attenuated mutants of this strain would induce altered expression of the newly identified fibroblast genes associated with immune and non-immune functions of CEFs. Gene expression was evaluated by real-time PCR following challenge by the parental strain SE 11 and its virulence attenuated mutants lacking flagellin gene fliD only or fliD and the serovar-specific virulence plasmid pSEV. As a result, deletion mutants induced a lower expression of all immune genes, but an increased expression of the non-immune genes G0S2 and ENO2 relative to the parental strain. Our data indicate the importance of flagella and pSEV in modulation of virulence and host response in this model. We demonstrated, for the first time ever, an increased induction of survival genes G0S2 and ENO2 by virulence-attenuated mutants of S. Enteritidis.
Citace poskytuje Crossref.org
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