Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Historic and geographic surveillance of Pseudogymnoascus destructans possible from collections of bat parasites

A. Zahradníková, V. Kovacova, N. Martínková, MV. Orlova, OL. Orlov, V. Piacek, J. Zukal, J. Pikula,

. 2018 ; 65 (2) : 303-308. [pub] 20171127

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033516

Specimens archived in wet collections represent valuable material for scientific research. Here, we show that bat fly (Diptera, Nycteribiidae) samples contain DNA of Pseudogymnoascus destructans, a fungus pathogenic to bats. Using dual-probe quantitative PCR, we detected P. destructans DNA on bat flies collected in the Samara, Sverdlovsk and Irkutsk regions of Russia between 2005 and 2017. Fungal load was significantly lower on bat flies from wet collections than on freshly collected mites in the Czech Republic. The bat pathogen was present in the Samara region (European part of Russia) in 2005, that is, a year before recognition of white-nose syndrome in North America. As Samara and Irkutsk regions were identified as new positive locations of P. destructans, our data expand the known geographic distribution of P. destructans. We conclude that ethanol-stored ectoparasites can be used to identify the presence of pathogens in historic bat populations and understudied geographical regions.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18033516
003      
CZ-PrNML
005      
20190222094958.0
007      
ta
008      
181008s2018 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1111/tbed.12773 $2 doi
035    __
$a (PubMed)29181887
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Zahradníková, A $u Department of Muscle Cell Research, Centre of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovakia.
245    10
$a Historic and geographic surveillance of Pseudogymnoascus destructans possible from collections of bat parasites / $c A. Zahradníková, V. Kovacova, N. Martínková, MV. Orlova, OL. Orlov, V. Piacek, J. Zukal, J. Pikula,
520    9_
$a Specimens archived in wet collections represent valuable material for scientific research. Here, we show that bat fly (Diptera, Nycteribiidae) samples contain DNA of Pseudogymnoascus destructans, a fungus pathogenic to bats. Using dual-probe quantitative PCR, we detected P. destructans DNA on bat flies collected in the Samara, Sverdlovsk and Irkutsk regions of Russia between 2005 and 2017. Fungal load was significantly lower on bat flies from wet collections than on freshly collected mites in the Czech Republic. The bat pathogen was present in the Samara region (European part of Russia) in 2005, that is, a year before recognition of white-nose syndrome in North America. As Samara and Irkutsk regions were identified as new positive locations of P. destructans, our data expand the known geographic distribution of P. destructans. We conclude that ethanol-stored ectoparasites can be used to identify the presence of pathogens in historic bat populations and understudied geographical regions.
650    _2
$a zvířata $7 D000818
650    _2
$a členovci - vektory $x mikrobiologie $7 D001179
650    _2
$a Ascomycota $x genetika $x izolace a purifikace $x patogenita $7 D001203
650    _2
$a Chiroptera $x parazitologie $7 D002685
650    _2
$a Česká republika $x epidemiologie $7 D018153
650    _2
$a DNA fungální $x genetika $7 D004271
650    _2
$a Diptera $x mikrobiologie $7 D004175
650    _2
$a infestace ektoparazity $x epidemiologie $7 D004478
650    _2
$a zeměpis $7 D005843
650    _2
$a mykózy $x epidemiologie $7 D009181
650    _2
$a parazitární zátěž $7 D059208
650    _2
$a kvantitativní polymerázová řetězová reakce $7 D060888
650    _2
$a Rusko $x epidemiologie $7 D012426
650    _2
$a uchovávání tkání $7 D014021
655    _2
$a časopisecké články $7 D016428
700    1_
$a Seidlová, Veronika $7 xx0267284 $u Department of Ecology and Diseases of Game, Fish and Bees, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Martínková, N $u Institute of Vertebrate Biology, Czech Academy of Sciences, Brno, Czech Republic. Institute of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic.
700    1_
$a Orlova, M V $u International Complex Research Laboratory for Study of Climate Change, Land Use and Biodiversity, Tyumen State University, Tyumen, Russia. Laboratory of Biodiversity Monitoring, National Research Tomsk State University, Tomsk, Russia.
700    1_
$a Orlov, O L $u International Complex Research Laboratory for Study of Climate Change, Land Use and Biodiversity, Tyumen State University, Tyumen, Russia. Department of Biochemistry, Ural State Medical University, Ekaterinburg, Russia.
700    1_
$a Piacek, V $u Department of Ecology and Diseases of Game, Fish and Bees, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
700    1_
$a Zukal, Jan, $d 1967- $7 ola2003204907 $u Institute of Vertebrate Biology, Czech Academy of Sciences, Brno, Czech Republic. Department of Botany and Zoology, Masaryk University, Brno, Czech Republic.
700    1_
$a Pikula, J $u Department of Ecology and Diseases of Game, Fish and Bees, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
773    0_
$w MED00169643 $t Transboundary and emerging diseases $x 1865-1682 $g Roč. 65, č. 2 (2018), s. 303-308
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29181887 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20181008 $b ABA008
991    __
$a 20190222095256 $b ABA008
999    __
$a ok $b bmc $g 1340187 $s 1030510
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 65 $c 2 $d 303-308 $e 20171127 $i 1865-1682 $m Transboundary and emerging diseases $n Transbound Emerg Dis $x MED00169643
LZP    __
$a Pubmed-20181008

Najít záznam

Citační ukazatele

Možnosti archivace