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Berbamine protects the heart from isoproterenol induced myocardial infarction by modulating eNOS and iNOS expressions in rats
Saranya Sithuraj, Vijaya Padma Viswanadha
Language English Country Czech Republic
Document type Research Support, Non-U.S. Gov't
- MeSH
- Benzylisoquinolines administration & dosage pharmacology MeSH
- Myocardial Infarction drug therapy chemically induced physiopathology MeSH
- Isoproterenol administration & dosage adverse effects MeSH
- Cardiotonic Agents MeSH
- Disease Models, Animal MeSH
- Nitrosative Stress drug effects MeSH
- Polymerase Chain Reaction methods MeSH
- Rats, Wistar MeSH
- Nitric Oxide Synthase Type II drug effects MeSH
- Nitric Oxide Synthase Type III drug effects MeSH
- In Vitro Techniques MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
The current study was designed to investigate the effect of berbamine (BBM) on isoproterenol (ISO) induced changes in cardiac marker enzymes, myocardial oxidative stress, lipid profile and expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in male Wistar rats. Rats were pretreated with BBM (25 mg/kg) through intraperitoneal injection for 7 days followed by induction of myocardial infarction (MI) by subcutaneous injection of ISO (85 mg/kg) for last two days. Key findings: In the present study, the histopathological findings of the heart tissue showed that BBM treatment significantly minimized the damage induced by ISO. BBM pretreatment showed a significant decrease in heart weight, serum marker enzymes, lipid peroxidation and significant increase in cardiac endogenous enzymatic and non-enzymatic antioxidants compared to the ISO-treated group. In addition, we observed significantly upregulated eNOS expression and downregulated iNOS expression in BBM pretreated group. Thus, BBM protected the rat’s heart from ISO-induced myocardial infarction by its antioxidant, and antilipidemic properties. Significance: The results of the present investigation suggested that BBM efficiently ameliorated the ISO-induced myocardial infarction in rats.
Asia University Department of Health and Nutrition Biotechnology Taichung Taiwan
Bharathiar University Department of Biotechnology Translational Research Laboratory Tamil Nadu India
China Medical University Graduate Institute of Basic Medical Science Taichung Taiwan
References provided by Crossref.org
Literatura
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- $a The current study was designed to investigate the effect of berbamine (BBM) on isoproterenol (ISO) induced changes in cardiac marker enzymes, myocardial oxidative stress, lipid profile and expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in male Wistar rats. Rats were pretreated with BBM (25 mg/kg) through intraperitoneal injection for 7 days followed by induction of myocardial infarction (MI) by subcutaneous injection of ISO (85 mg/kg) for last two days. Key findings: In the present study, the histopathological findings of the heart tissue showed that BBM treatment significantly minimized the damage induced by ISO. BBM pretreatment showed a significant decrease in heart weight, serum marker enzymes, lipid peroxidation and significant increase in cardiac endogenous enzymatic and non-enzymatic antioxidants compared to the ISO-treated group. In addition, we observed significantly upregulated eNOS expression and downregulated iNOS expression in BBM pretreated group. Thus, BBM protected the rat’s heart from ISO-induced myocardial infarction by its antioxidant, and antilipidemic properties. Significance: The results of the present investigation suggested that BBM efficiently ameliorated the ISO-induced myocardial infarction in rats.
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