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41 záznamů v Medvik Filtry

Článek

Berbamine protects the heart from isoproterenol induced myocardial infarction by modulating eNOS and iNOS expressions in rats

Sithuraj, Saranya
Autor Sithuraj, Saranya Bharathiar University, Department of Biotechnology, Translational Research Laboratory, Tamil Nadu, India
Viswanadha, Vijaya Padma
Autor Viswanadha, Vijaya Padma Bharathiar University, Department of Biotechnology, Translational Research Laboratory, Tamil Nadu, India China Medical University, Graduate Institute of Basic Medical Science, Taichung, Taiwan Asia University, Department of Health and Nutrition Biotechnology, Taichung, Taiwan

Journal of Applied Biomedicine. 2018 ; 16 (4) : 301-310.

ISSN 1214-021X
Medvik
Zdroj

The current study was designed to investigate the effect of berbamine (BBM) on isoproterenol (ISO) induced changes in cardiac marker enzymes, myocardial oxidative stress, lipid profile and expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in male Wistar rats. Rats were pretreated with BBM (25 mg/kg) through intraperitoneal injection for 7 days followed by induction of myocardial infarction (MI) by subcutaneous injection of ISO (85 mg/kg) for last two days. Key findings: In the present study, the histopathological findings of the heart tissue showed that BBM treatment significantly minimized the damage induced by ISO. BBM pretreatment showed a significant decrease in heart weight, serum marker enzymes, lipid peroxidation and significant increase in cardiac endogenous enzymatic and non-enzymatic antioxidants compared to the ISO-treated group. In addition, we observed significantly upregulated eNOS expression and downregulated iNOS expression in BBM pretreated group. Thus, BBM protected the rat’s heart from ISO-induced myocardial infarction by its antioxidant, and antilipidemic properties. Significance: The results of the present investigation suggested that BBM efficiently ameliorated the ISO-induced myocardial infarction in rats.

Kapitola

Dogoxin, inotropní látky a symptomimetika

Farmakoterapie kardiovaskulárních onemocnění. 2017 ; () : 83-96.

ISBN 978-80-247-4713-2
Medvik
Zdroj

Článek

Intravenous rutin in rat exacerbates isoprenaline-induced cardiotoxicity likely due to intracellular oxidative stress

Redox report. 2017 ; 22 (2) : 78-90. [pub] 20160321

Redox Rep
ISSN 1351-0002
Medvik
Zdroj

OBJECTIVES: Rutin, quercetin-3-O-rutinoside, a natural flavonol glycoside, has shown various in vitro benefits with potential use treating human diseases, especially cardiovascular system disorders. Antioxidant properties are assumed to underlie the majority of these benefits. Yet rutin pro-oxidant properties have been reported as well. Our research group has recently shown aggravating effects on isoprenaline (ISO)-induced cardiotoxicity in Wistar:Han rats after 24 hours. METHODS: This study was designed to examine in more detail the reasons for the negative effects of rutin (11.5 and 46 mg/kg, i.v.) after administration of ISO (100 mg/kg, s.c.) in rats within 2 hours of continuous experiment and in the H9c2 cardiomyoblast-derived cell line. RESULTS: Like our previous findings, rutin did not (11.5 or 46 mg/kg, i.v.) reduce the ISO-induced mortality within 2 hours although the lower dose significantly reduced cardiac troponin T (cTnT) and partly improved the histological findings. In contrast, the higher dose increased the mortality in comparison with solvent (1.26% w/v sodium bicarbonate). This was not caused by any specific haemodynamic disturbances. It appears to be associated with oxidative stress as rutin enhanced intracellular reactive oxygen species formation in vitro and had the tendency to increase it in vivo. CONCLUSIONS: Rutin, likely due to its pro-oxidative effects, can exacerbate catecholamine cardiotoxicity depending on the dose used.