The current study was designed to investigate the effect of berbamine (BBM) on isoproterenol (ISO) induced changes in cardiac marker enzymes, myocardial oxidative stress, lipid profile and expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in male Wistar rats. Rats were pretreated with BBM (25 mg/kg) through intraperitoneal injection for 7 days followed by induction of myocardial infarction (MI) by subcutaneous injection of ISO (85 mg/kg) for last two days. Key findings: In the present study, the histopathological findings of the heart tissue showed that BBM treatment significantly minimized the damage induced by ISO. BBM pretreatment showed a significant decrease in heart weight, serum marker enzymes, lipid peroxidation and significant increase in cardiac endogenous enzymatic and non-enzymatic antioxidants compared to the ISO-treated group. In addition, we observed significantly upregulated eNOS expression and downregulated iNOS expression in BBM pretreated group. Thus, BBM protected the rat’s heart from ISO-induced myocardial infarction by its antioxidant, and antilipidemic properties. Significance: The results of the present investigation suggested that BBM efficiently ameliorated the ISO-induced myocardial infarction in rats.
- MeSH
- benzylisochinoliny aplikace a dávkování farmakologie MeSH
- infarkt myokardu farmakoterapie chemicky indukované patofyziologie MeSH
- isoprenalin aplikace a dávkování škodlivé účinky MeSH
- kardiotonika MeSH
- modely nemocí na zvířatech MeSH
- nitrosativní stres účinky léků MeSH
- polymerázová řetězová reakce metody MeSH
- potkani Wistar MeSH
- synthasa oxidu dusnatého, typ II účinky léků MeSH
- synthasa oxidu dusnatého, typ III účinky léků MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
