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Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept
D. Schwarz, AKJ. Schoenenwald, J. Dörrstein, J. Sterba, D. Kahoun, P. Fojtíková, J. Vilímek, D. Schieder, C. Zollfrank, V. Sieber,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
- MeSH
- bioreaktory MeSH
- Cupriavidus necator MeSH
- fermentace * MeSH
- hydroxybutyráty metabolismus MeSH
- lipnicovité * MeSH
- polyestery metabolismus MeSH
- siláž MeSH
- Publikační typ
- časopisecké články MeSH
Grass silage as a renewable feedstock for an integrated biorefinery includes nutrients and carbon sources directly available in the press juice (PJ) and in lignocellulosic saccharides from the plant framework. Here, a novel two-stage fed-batch fermentation process for biosynthesis of poly-3-hydroxybutyrate (PHB) by Cupriavidus necator DSM 531 is presented. For bacterial growth, nutrient-rich PJ was employed as a fermentation medium, without any supplements. Saccharides derived from the mechano-enzymatic hydrolysis of the press cake (PC) were subjected to a lactic acid fermentation process, before the fermentation products were fed into the polymer accumulation phase. By combination of pH-stat feeding and cell recycling, the PHB content in 22 g L-1 total-dry cells reached 39% after 32 h of cultivation. Using mimicked hydrolyzate of diluted PJ artificially supplemented with glucose and xylose, the resulting cell dry weight of 21 g L-1 contained 42% PHB.
Citace poskytuje Crossref.org
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