Helicobacter pylori (H. pylori) can convert to coccoid form in unfavorable conditions or as a result of antibiotic treatment. In order to adapt to harsh environments, H. pylori requires a stringent response which, encoded by the spoT gene, has a bifunctional enzyme possessing both (p)ppGpp synthetic and degrading activity. Our goal in this study was to compare spoT gene expression in spiral and induced coccoid forms of H. pylori with use of amoxicillin. First, clinical isolate coccoid forms were induced with amoxicillin; then, the viability test was analyzed by flow cytometer. After RNA extraction, cDNA synthesis and designing a specific primer for spoT gene, evaluation of the desired gene expression in both forms were studied. Bacterial isolates exposed to amoxicillin at MIC and 1/2 MIC induced morphological conversion better and faster than other MIC concentration. The expression of spoT gene was significantly downregulated in spiral forms of H. pylori, while the gene expression was upregulated and + 30.3-fold changes was seen in coccoid forms of bacterium. To summarize, spoT gene is one of the key factors for antibiotic resistance and its enhanced expression in coccoid form can be a valuable diagnostic marker for recognition of H. pylori during morphological conversion.

Department of Microbiology Isfahan University of Medical Sciences Isfahan Iran

Department of Microbiology Tonekabon Branch Islamic Azad University Tonekabon Iran

000      

00000naa a2200000 a 4500

001      

bmc19002798

003      

CZ-PrNML

005      

20190116123315.0

007      

ta

008      

190116s2018 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1007/s12223-017-0557-0 $2 doi

035    __

$a (PubMed)29327293

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Poursina, Farkhondeh $u Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.

245    10

$a Overexpression of spoT gene in coccoid forms of clinical Helicobacter pylori isolates / $c F. Poursina, J. Fagri, N. Mirzaei, HG. Safaei,

520    9_

$a Helicobacter pylori (H. pylori) can convert to coccoid form in unfavorable conditions or as a result of antibiotic treatment. In order to adapt to harsh environments, H. pylori requires a stringent response which, encoded by the spoT gene, has a bifunctional enzyme possessing both (p)ppGpp synthetic and degrading activity. Our goal in this study was to compare spoT gene expression in spiral and induced coccoid forms of H. pylori with use of amoxicillin. First, clinical isolate coccoid forms were induced with amoxicillin; then, the viability test was analyzed by flow cytometer. After RNA extraction, cDNA synthesis and designing a specific primer for spoT gene, evaluation of the desired gene expression in both forms were studied. Bacterial isolates exposed to amoxicillin at MIC and 1/2 MIC induced morphological conversion better and faster than other MIC concentration. The expression of spoT gene was significantly downregulated in spiral forms of H. pylori, while the gene expression was upregulated and + 30.3-fold changes was seen in coccoid forms of bacterium. To summarize, spoT gene is one of the key factors for antibiotic resistance and its enhanced expression in coccoid form can be a valuable diagnostic marker for recognition of H. pylori during morphological conversion.

650    _2

$a fyziologická adaptace $x genetika $7 D000222

650    _2

$a amoxicilin $x metabolismus $x farmakologie $7 D000658

650    _2

$a antibakteriální látky $x metabolismus $x farmakologie $7 D000900

650    _2

$a bakteriální léková rezistence $x genetika $7 D024881

650    _2

$a regulace genové exprese u bakterií $x účinky léků $x fyziologie $7 D015964

650    _2

$a bakteriální geny $x genetika $7 D005798

650    _2

$a Helicobacter pylori $x účinky léků $x genetika $x růst a vývoj $7 D016480

650    _2

$a mikrobiální testy citlivosti $7 D008826

650    _2

$a mikrobiální viabilita $x účinky léků $7 D050296

650    _2

$a pyrofosfatasy $x genetika $7 D011755

655    _2

$a časopisecké články $7 D016428

700    1_

$a Fagri, Jamshid $u Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.

700    1_

$a Mirzaei, Nasrin $u Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.

700    1_

$a Safaei, Hajieh Ghasemian $u Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran. ghasemian@med.mui.ac.ir.

773    0_

$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 63, č. 4 (2018), s. 459-465

856    41

$u https://pubmed.ncbi.nlm.nih.gov/29327293 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20190116 $b ABA008

991    __

$a 20190116123527 $b ABA008

999    __

$a ok $b bmc $g 1367975 $s 1040956

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2018 $b 63 $c 4 $d 459-465 $e 20180111 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005

LZP    __

$a Pubmed-20190116