Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Expression of BCL-2 in the developing kidney of human embryos and fetuses qualitative and quantitative study

V. Lichnovský, B. Erdösová, K. Punkt, M. Zapletal

. 1999 ; 142 (142) : 61-64.

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19007712

Twelve human embryos and fetuses aged of 7-30 weeks of intrauterinal life were examined to determine the expression of bcl-2 gene in the developing kidney. Tissue samples were routinely processed and three-step indirect immunohistochemical method was used for the detection of Bcl-2 protein. End-point cytophotometry was performed with computer-controlled microscope photometer with a scanning table and the mean relative absorbance of the final product of peroxidase reaction was determined and taken as a measure of Bcl-2 expression. The morphometric evaluation was carried out from the TV display using Weibel s universal hexagonal raster and we determined the relative volume of Bcl-2 positive structures in the various zones of the embryonal kidney. The aim of our research was mapping of the Bcl-2 occurrence in the developing kidney of human embryos and fetuses. The Bcl-2 protein is involved in the regulation of apoptosis and its effect is antiapoptotic. The highest Bcl-2 expression was proved in the cells of metanephrogenic blastema. The lower occurrence of Bcl-2 positive cells was demonstrated in proximal tubules analges+ and it was almost on the borderline of detection in branches of ureteral bud. In the fetal period the marked Bcl-2 expression was maintained in the epithelial cells of proximal tubules analges.

000      
00000naa a2200000 a 4500
001      
bmc19007712
003      
CZ-PrNML
005      
20190403105143.0
007      
ta
008      
190227s1999 xr ad f 000 0|eng||
009      
AR
035    __
$a (PubMed)10743726
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Lichnovský, Václav, $u Institute of Histology and Embryology, Faculty of Medicine, Palacký University, Olomouc, Czech Republic $d 1941- $7 ola2003167501
245    10
$a Expression of BCL-2 in the developing kidney of human embryos and fetuses qualitative and quantitative study / $c V. Lichnovský, B. Erdösová, K. Punkt, M. Zapletal
520    9_
$a Twelve human embryos and fetuses aged of 7-30 weeks of intrauterinal life were examined to determine the expression of bcl-2 gene in the developing kidney. Tissue samples were routinely processed and three-step indirect immunohistochemical method was used for the detection of Bcl-2 protein. End-point cytophotometry was performed with computer-controlled microscope photometer with a scanning table and the mean relative absorbance of the final product of peroxidase reaction was determined and taken as a measure of Bcl-2 expression. The morphometric evaluation was carried out from the TV display using Weibel s universal hexagonal raster and we determined the relative volume of Bcl-2 positive structures in the various zones of the embryonal kidney. The aim of our research was mapping of the Bcl-2 occurrence in the developing kidney of human embryos and fetuses. The Bcl-2 protein is involved in the regulation of apoptosis and its effect is antiapoptotic. The highest Bcl-2 expression was proved in the cells of metanephrogenic blastema. The lower occurrence of Bcl-2 positive cells was demonstrated in proximal tubules analges+ and it was almost on the borderline of detection in branches of ureteral bud. In the fetal period the marked Bcl-2 expression was maintained in the epithelial cells of proximal tubules analges.
650    _2
$a cytofotometrie $7 D003592
650    _2
$a embryo savčí $7 D004622
650    12
$a embryonální a fetální vývoj $7 D005314
650    _2
$a plod $7 D005333
650    12
$a vývojová regulace genové exprese $7 D018507
650    12
$a geny bcl-2 $7 D019254
650    _2
$a gestační stáří $7 D005865
650    _2
$a lidé $7 D006801
650    _2
$a ledviny $x cytologie $x embryologie $7 D007668
650    _2
$a kůra ledviny $x embryologie $7 D007672
650    _2
$a dřeň ledvin $x embryologie $7 D007679
650    _2
$a protoonkogenní proteiny c-bcl-2 $x analýza $7 D019253
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Erdösová, Běla, $d 1956- $7 xx0019004 $u Institute of Histology and Embryology, Faculty of Medicine, Palacký University, Olomouc, Czech Republic
700    1_
$a Punkt, Karla $u Institute of Anatomy, University of Leipzig, Germany
700    1_
$a Zapletal, Michal $7 _AN100604 $u Institute of Histology and Embryology, Faculty of Medicine, Palacký University, Olomouc, Czech Republic
773    0_
$w MED00011326 $t Acta Universitatis Palackianae Olomucensis Facultatis Medicae $x 0862-481X $g Roč. 142, č. 142 (1999), s. 61-64
856    41
$u https://pubmed.ncbi.nlm.nih.gov/10743726 $y Pubmed
856    41
$u https://biomed.papers.upol.cz/pdfs/bio/1999/01/10.pdf $y plný text volně přístupný
910    __
$a ABA008 $b A 1502 $y a $z 0
990    __
$a 20190227 $b ABA008
991    __
$a 20190403105149 $b ABA008
999    __
$a ok $b bmc $g 1380196 $s 1045958
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 1999 $b 142 $c 142 $d 61-64 $i 0862-481X $m Acta Universitatis Palackianae Olomucensis Facultatis medicae. Biomedical papers $n Acta Univ. Palacki. Olomuc. Fac. med., Biomed. Pap. $x MED00011326
LZP    __
$b NLK118 $a Pubmed-20190227

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...