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The elicitin β-cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions
T. Starý, P. Satková, J. Piterková, B. Mieslerová, L. Luhová, J. Mikulík, T. Kašparovský, M. Petřivalský, J. Lochman,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
NLK
ProQuest Central
od 2002-11-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 1999-11-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2002-11-01 do Před 1 rokem
- MeSH
- cyklopentany metabolismus MeSH
- ethyleny metabolismus MeSH
- fungální proteiny metabolismus MeSH
- interakce hostitele a patogenu MeSH
- kyselina salicylová metabolismus MeSH
- listy rostlin metabolismus mikrobiologie MeSH
- nemoci rostlin mikrobiologie MeSH
- oxylipiny metabolismus MeSH
- peroxid vodíku metabolismus MeSH
- Phytophthora MeSH
- Pythium MeSH
- reaktivní formy kyslíku metabolismus MeSH
- regulátory růstu rostlin metabolismus MeSH
- signální transdukce * MeSH
- Solanum lycopersicum metabolismus mikrobiologie fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
MAIN CONCLUSION: The level of resistance induced in different tomato genotypes after β-CRY treatment correlated with the upregulation of defence genes, but not sterol binding and involved ethylene and jasmonic acid signalling. Elicitins, a family of small proteins secreted by Phytophthora and Pythium spp., are the most well-known microbe-associated molecular patterns of oomycetes, a lineage of fungus-like organisms that include many economically significant crop pathogens. The responses of tomato plants to elicitin INF1 produced by Phytophthora infestans have been studied extensively. Here, we present studies on the responses of three tomato genotypes to β-cryptogein (β-CRY), a potent elicitin secreted by Phytophthora cryptogea that induces hypersensitive response (HR) cell death in tobacco plants and confers greater resistance to oomycete infection than acidic elicitins like INF1. We also studied β-CRY mutants impaired in sterol binding (Val84Phe) and interaction with the binding site on tobacco plasma membrane (Leu41Phe), because sterol binding was suggested to be important in INF1-induced resistance. Treatment with β-CRY or the Val84Phe mutant induced resistance to powdery mildew caused by the pathogen Pseudoidium neolycopersici, but not the HR cell death observed in tobacco and potato plants. The level of resistance induced in different tomato genotypes correlated with the upregulation of defence genes including defensins, β-1,3-glucanases, heveins, chitinases, osmotins, and PR1 proteins. Treatment with the Leu41Phe mutant did not induce this upregulation, suggesting similar elicitin recognition in tomato and tobacco. However, here β-CRY activated ethylene and jasmonic acid signalling, but not salicylic acid signalling, demonstrating that elicitins activate different downstream signalling processes in different plant species. This could potentially be exploited to enhance the resistance of Phytophthora-susceptible crops.
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- $a Starý, Tomáš $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137, Brno, Czech Republic.
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- $a The elicitin β-cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions / $c T. Starý, P. Satková, J. Piterková, B. Mieslerová, L. Luhová, J. Mikulík, T. Kašparovský, M. Petřivalský, J. Lochman,
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- $a MAIN CONCLUSION: The level of resistance induced in different tomato genotypes after β-CRY treatment correlated with the upregulation of defence genes, but not sterol binding and involved ethylene and jasmonic acid signalling. Elicitins, a family of small proteins secreted by Phytophthora and Pythium spp., are the most well-known microbe-associated molecular patterns of oomycetes, a lineage of fungus-like organisms that include many economically significant crop pathogens. The responses of tomato plants to elicitin INF1 produced by Phytophthora infestans have been studied extensively. Here, we present studies on the responses of three tomato genotypes to β-cryptogein (β-CRY), a potent elicitin secreted by Phytophthora cryptogea that induces hypersensitive response (HR) cell death in tobacco plants and confers greater resistance to oomycete infection than acidic elicitins like INF1. We also studied β-CRY mutants impaired in sterol binding (Val84Phe) and interaction with the binding site on tobacco plasma membrane (Leu41Phe), because sterol binding was suggested to be important in INF1-induced resistance. Treatment with β-CRY or the Val84Phe mutant induced resistance to powdery mildew caused by the pathogen Pseudoidium neolycopersici, but not the HR cell death observed in tobacco and potato plants. The level of resistance induced in different tomato genotypes correlated with the upregulation of defence genes including defensins, β-1,3-glucanases, heveins, chitinases, osmotins, and PR1 proteins. Treatment with the Leu41Phe mutant did not induce this upregulation, suggesting similar elicitin recognition in tomato and tobacco. However, here β-CRY activated ethylene and jasmonic acid signalling, but not salicylic acid signalling, demonstrating that elicitins activate different downstream signalling processes in different plant species. This could potentially be exploited to enhance the resistance of Phytophthora-susceptible crops.
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- $a Satková, Pavla $u Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech Republic.
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- $a Mieslerová, Barbora $u Department of Botany, Faculty of Science, Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech Republic.
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- $a Luhová, Lenka $u Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech Republic.
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- $a Mikulík, Jaromír $u Laboratory of Growth Regulators, Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Institute of Experimental Botany ASCR, Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech Republic.
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- $a Kašparovský, Tomáš $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137, Brno, Czech Republic.
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- $a Petřivalský, Marek $u Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech Republic.
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- $a Lochman, Jan $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137, Brno, Czech Republic. lochik@mail.muni.cz.
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