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Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models

G. Caluori, J. Pribyl, M. Pesl, S. Jelinkova, V. Rotrekl, P. Skladal, R. Raiteri,

. 2019 ; 124-125 (-) : 129-135. [pub] 20181016

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc19012219

Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric / nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs' drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The β-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model.

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$a Caluori, Guido $u Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic; International Clinical Research Centre of Saint Anne Hospital Brno, Pekarska 53, 60200 Brno, Czech Republic; Department of Informatics, Bioengineering Robotics and Systems Engineering, University of Genova, Via All'Opera Pia, 13, 16145 Genova, Italy.
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$a Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models / $c G. Caluori, J. Pribyl, M. Pesl, S. Jelinkova, V. Rotrekl, P. Skladal, R. Raiteri,
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$a Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric / nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs' drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The β-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model.
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$a Pribyl, Jan $u Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
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$a Pesl, Martin $u International Clinical Research Centre of Saint Anne Hospital Brno, Pekarska 53, 60200 Brno, Czech Republic; Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic; 1st Department of Cardiovascular Diseases, St. Anne's University Hospital and Masaryk University, Pekarska 53, Brno, Czech Republic.
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$a Jelinkova, Sarka $u Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
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$a Rotrekl, Vladimir $u Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
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$a Skladal, Petr $u Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic. Electronic address: skladal@chemi.muni.cz.
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$a Raiteri, Roberto $u Department of Informatics, Bioengineering Robotics and Systems Engineering, University of Genova, Via All'Opera Pia, 13, 16145 Genova, Italy. Electronic address: roberto.raiteri@unige.it.
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