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Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models
G. Caluori, J. Pribyl, M. Pesl, S. Jelinkova, V. Rotrekl, P. Skladal, R. Raiteri,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
- MeSH
- biosenzitivní techniky * MeSH
- buněčná diferenciace genetika MeSH
- Duchennova muskulární dystrofie patofyziologie MeSH
- dystrofin genetika MeSH
- fibroblasty účinky léků ultrastruktura MeSH
- indukované pluripotentní kmenové buňky metabolismus ultrastruktura MeSH
- isoprenalin farmakologie MeSH
- kardiomyocyty cytologie MeSH
- kontrakce myokardu genetika fyziologie MeSH
- lidé MeSH
- mikroelektrody MeSH
- mikroskopie atomárních sil MeSH
- verapamil farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric / nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs' drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The β-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model.
Central European Institute of Technology Masaryk University Kamenice 5 62500 Brno Czech Republic
Department of Biology Faculty of Medicine Masaryk University Kamenice 5 62500 Brno Czech Republic
Citace poskytuje Crossref.org
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