Background: The present study focused on the production of L-asparaginase using Solid State Fermentation (SSF) by Myroides gitamensis. Methods: Initially, five significant parameters (Carbon source; Nitrogen source, temperature, pH and incubation period) were identified that affect the production process of L-asparaginase using Classical One Factor at a Time (OFAT) optimization. An optimized L-asparaginase specific activity obtained by OFAT was recorded as 85.7 IU. Central Composite Design (CCD) was also employed successively to optimize the multiple parameters at a time and their results were compared. Results: Maximum L-asparaginase enzyme specific activity obtained by CCD method was 295.6 IU under the hold values of carbon source (wheat bran) 12 g/L, nitrogen source (yeast extract) 7 g/L, temperature 37°C, pH=7.5 and incubation period 47 hr. Upon validation, the obtained results proved that there was a good relation existing between the experimental and the predicted model (p<0.05). L-asparaginase activity was enhanced in statistical method up to 3.4 folds compared to that of classical method. Conclusion: Utilization of wheat bran as a low cost carbon source in SSF for the production of L-asparaginase enzyme makes the process economical and in turn reduces the environmental pollution by biotransformation to commercially useful bio product.

Department of Biotechnology College of Natural and Computational Science University of Gondar Gondar Ethiopia Department of Biotechnology GITAM Institute of Science GITAM University Visakhapatnam 530 045 Andhra Pradesh India Department of Biotechnology University of Chemistry and Technology Prague Technicka 5 CZ 16628 Prague 6 Czech Republic

Department of Biotechnology GITAM Institute of Science GITAM University Visakhapatnam 530 045 Andhra Pradesh India