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Combintion of atomic force microscopy and comet assay for analysis of DNA damage induced by PDT
Hana Zapletalová, Kateřina Bartoň Tománková, Jakub Malohlava, Milan Vůjtek, Hana Kolářová
Lékař a technika.
2016 ;
46 (4) :
83-90.
Language English Country Czech Republic
Persistent link
https://www.medvik.cz/link/bmc19015228
- MeSH
- DNA radiation effects MeSH
- Photochemotherapy * adverse effects MeSH
- Photosensitizing Agents adverse effects MeSH
- Comet Assay methods MeSH
- Humans MeSH
- Microscopy, Atomic Force * methods MeSH
- DNA Damage * radiation effects MeSH
- Dose-Response Relationship, Drug MeSH
- Check Tag
- Humans MeSH
The aim of the present study was to evaluate the efficiency of photosensitisation induced by two photosensitizers, TMPyP and ClAlPcS2, tested in vitro on the tumor cell line MCF7. The oxidative damage of DNA in MCF-7 cells was analyzed by comet assay (CA) combined with Atomic Force Microscopy (AFM). The ability of detection of apoptotic response detected by Atomic Force Microscopy at the individual molecule level of DNA was successfully demonstrated; when DNA get damaged, cleavage to fragments caused by photodynamic treatment was directly visualized by AFM imaging of individual molecules. Its accuracy and reliability was validated through the comparison with traditional single cell agarose electrophoresis.
Bibliography, etc.
Literatura
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