PURPOSE: Crohn's disease (CD) belongs to chronic disorders with unpredictable disease course. The aim of this study was to identify how genetic testing (NOD2/CARD15) can be used in patients with CD to predict the need for surgical treatment (to define an aggressive type of disease where the patient can profit from early surgery). METHODS: The patients who were tested genetically had undergone a surgery due to CD at the Department of Surgery University Hospital Brno Bohunice between 2010 and 2016. The control group consisted of patients with CD who had been diagnosed with CD at least 5 years prior to the testing and had not required any surgical intervention. The second control group was healthy subjects. RESULTS: In total, there were 117 operated patients for CD, 77 patients with CD that had not undergone surgery for CD and 30 healthy subjects. For patients with at least one genetic mutation, the risk of the necessity of surgical treatment of CD is 1.96 times higher than for patients with no mutation. Patients with two or more mutations were generally operated on at a younger age, in a shorter time after being diagnosed and each patient had a partial resection of the ileum. CONCLUSION: The group of operated patients with CD had a significantly higher distribution of at least one genetic mutation as opposed to the non-operated group. In patients with two or more mutations, the disease course was more aggressive. This group of patients might profit from the conservative top-down or early surgical therapy.

Department of Gastroenterology University Hospital Brno Bohunice Faculty of Medicine Masaryk University Brno Czech Republic

Department of Surgery University Hospital Brno Bohunice Faculty of Medicine Masaryk University Jihlavska 20 62500 Brno Czech Republic

Department of Surgery University Hospital Brno Bohunice Faculty of Medicine Masaryk University Jihlavska 20 62500 Brno Czech Republic Department of Gastroenterology University Hospital Brno Bohunice Faculty of Medicine Masaryk University Brno Czech Republic

Institute of Biostatistics and Analyses Faculty of Medicine Masaryk University Brno Czech Republic Institute of Health Information and Statistics of the Czech Republic Prague Czech Republic

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$a Kunovsky, L $u Department of Surgery, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Jihlavska 20, 62500, Brno, Czech Republic. Department of Gastroenterology, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

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$a The role of the NOD2/CARD15 gene in surgical treatment prediction in patients with Crohn's disease / $c L. Kunovsky, Z. Kala, F. Marek, J. Dolina, K. Poredska, L. Kucerova, K. Benesova, L. Mitas,

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$a PURPOSE: Crohn's disease (CD) belongs to chronic disorders with unpredictable disease course. The aim of this study was to identify how genetic testing (NOD2/CARD15) can be used in patients with CD to predict the need for surgical treatment (to define an aggressive type of disease where the patient can profit from early surgery). METHODS: The patients who were tested genetically had undergone a surgery due to CD at the Department of Surgery University Hospital Brno Bohunice between 2010 and 2016. The control group consisted of patients with CD who had been diagnosed with CD at least 5 years prior to the testing and had not required any surgical intervention. The second control group was healthy subjects. RESULTS: In total, there were 117 operated patients for CD, 77 patients with CD that had not undergone surgery for CD and 30 healthy subjects. For patients with at least one genetic mutation, the risk of the necessity of surgical treatment of CD is 1.96 times higher than for patients with no mutation. Patients with two or more mutations were generally operated on at a younger age, in a shorter time after being diagnosed and each patient had a partial resection of the ileum. CONCLUSION: The group of operated patients with CD had a significantly higher distribution of at least one genetic mutation as opposed to the non-operated group. In patients with two or more mutations, the disease course was more aggressive. This group of patients might profit from the conservative top-down or early surgical therapy.

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$a Kala, Z $u Department of Surgery, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Jihlavska 20, 62500, Brno, Czech Republic.

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$a Marek, F $u Department of Surgery, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Jihlavska 20, 62500, Brno, Czech Republic. fillip.marek@email.cz.

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$a Dolina, J $u Department of Gastroenterology, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

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$a Poredska, K $u Department of Gastroenterology, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

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$a Kucerova, L $u Department of Gastroenterology, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

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$a Benesova, K $u Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic. Institute of Health Information and Statistics of the Czech Republic, Prague, Czech Republic.

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$a Mitas, L $u Department of Surgery, University Hospital Brno Bohunice, Faculty of Medicine, Masaryk University, Jihlavska 20, 62500, Brno, Czech Republic.

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