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Ethanol fixation method for heart and lung imaging in micro-CT
M. Patzelt, J. Mrzilkova, J. Dudak, F. Krejci, J. Zemlicka, J. Karch, V. Musil, J. Rosina, V. Sykora, B. Horehledova, P. Zach,
Jazyk angličtina Země Japonsko
Typ dokumentu časopisecké články
Grantová podpora
GAUK 130717
Grantová Agentura, Univerzita Karlova
No. CZ.02.1.01/0.0/0.0/16_019/0000766
European Regional Development Fund-Project: Engineering applications of microworld physics
NLK
ProQuest Central
od 2009-01-01 do Před 1 rokem
CINAHL Plus with Full Text (EBSCOhost)
od 2011-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2011-01-01 do Před 1 rokem
Nursing & Allied Health Database (ProQuest)
od 2009-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2009-01-01 do Před 1 rokem
- MeSH
- ethanol * MeSH
- kontrastní látky * MeSH
- modely u zvířat MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- plíce anatomie a histologie diagnostické zobrazování MeSH
- rentgenová mikrotomografie metody MeSH
- srdce anatomie a histologie diagnostické zobrazování MeSH
- vylepšení obrazu metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
PURPOSE: The soft tissue imaging in micro-CT remains challenging due to its low intrinsic contrast. The aim of this study was to create a simple staining method omitting the usage of contrast agents for ex vivo soft tissue imaging in micro-CT. MATERIALS AND METHODS: Hearts and lungs from 30 mice were used. Twenty-seven organs were either fixed in 97% or 50% ethanol solution or in a series of ascending ethanol concentrations. Images were acquired after 72, 168 and 336 h on a custom-built micro-CT machine and compared to scans of three native samples. RESULTS: Ethanol provided contrast enhancement in all evaluated fixations. Fixation in 97% ethanol resulted in contrast enhancement after 72 h; however, it caused hardening of the samples. Fixation in 50% ethanol provided contrast enhancement after 336 h, with milder hardening, compared to the 97% ethanol fixation, but the visualization of details was worse. The fixation in a series of ascending ethanol concentrations provided the most satisfactory results; all organs were visualized in great detail without tissue damage. CONCLUSIONS: Simple ethanol fixation improves the tissue contrast enhancement in micro-CT. The best results can be obtained with fixation of the soft tissue samples in a series of ascending ethanol concentrations.
Citace poskytuje Crossref.org
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- $a PURPOSE: The soft tissue imaging in micro-CT remains challenging due to its low intrinsic contrast. The aim of this study was to create a simple staining method omitting the usage of contrast agents for ex vivo soft tissue imaging in micro-CT. MATERIALS AND METHODS: Hearts and lungs from 30 mice were used. Twenty-seven organs were either fixed in 97% or 50% ethanol solution or in a series of ascending ethanol concentrations. Images were acquired after 72, 168 and 336 h on a custom-built micro-CT machine and compared to scans of three native samples. RESULTS: Ethanol provided contrast enhancement in all evaluated fixations. Fixation in 97% ethanol resulted in contrast enhancement after 72 h; however, it caused hardening of the samples. Fixation in 50% ethanol provided contrast enhancement after 336 h, with milder hardening, compared to the 97% ethanol fixation, but the visualization of details was worse. The fixation in a series of ascending ethanol concentrations provided the most satisfactory results; all organs were visualized in great detail without tissue damage. CONCLUSIONS: Simple ethanol fixation improves the tissue contrast enhancement in micro-CT. The best results can be obtained with fixation of the soft tissue samples in a series of ascending ethanol concentrations.
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