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Ethanol fixation method for heart and lung imaging in micro-CT
M. Patzelt, J. Mrzilkova, J. Dudak, F. Krejci, J. Zemlicka, J. Karch, V. Musil, J. Rosina, V. Sykora, B. Horehledova, P. Zach,
Language English Country Japan
Document type Journal Article
Grant support
GAUK 130717
Grantová Agentura, Univerzita Karlova
No. CZ.02.1.01/0.0/0.0/16_019/0000766
European Regional Development Fund-Project: Engineering applications of microworld physics
NLK
ProQuest Central
from 2009-01-01 to 1 year ago
CINAHL Plus with Full Text (EBSCOhost)
from 2011-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2011-01-01 to 1 year ago
Nursing & Allied Health Database (ProQuest)
from 2009-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2009-01-01 to 1 year ago
- MeSH
- Ethanol * MeSH
- Contrast Media * MeSH
- Models, Animal MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Lung anatomy & histology diagnostic imaging MeSH
- X-Ray Microtomography methods MeSH
- Heart anatomy & histology diagnostic imaging MeSH
- Image Enhancement methods MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
PURPOSE: The soft tissue imaging in micro-CT remains challenging due to its low intrinsic contrast. The aim of this study was to create a simple staining method omitting the usage of contrast agents for ex vivo soft tissue imaging in micro-CT. MATERIALS AND METHODS: Hearts and lungs from 30 mice were used. Twenty-seven organs were either fixed in 97% or 50% ethanol solution or in a series of ascending ethanol concentrations. Images were acquired after 72, 168 and 336 h on a custom-built micro-CT machine and compared to scans of three native samples. RESULTS: Ethanol provided contrast enhancement in all evaluated fixations. Fixation in 97% ethanol resulted in contrast enhancement after 72 h; however, it caused hardening of the samples. Fixation in 50% ethanol provided contrast enhancement after 336 h, with milder hardening, compared to the 97% ethanol fixation, but the visualization of details was worse. The fixation in a series of ascending ethanol concentrations provided the most satisfactory results; all organs were visualized in great detail without tissue damage. CONCLUSIONS: Simple ethanol fixation improves the tissue contrast enhancement in micro-CT. The best results can be obtained with fixation of the soft tissue samples in a series of ascending ethanol concentrations.
References provided by Crossref.org
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- $a Patzelt, Matej $u Specialized Laboratory of Experimental Imaging, Third Faculty of Medicine, Charles University, Prague, Czech Republic. Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic. Department of Anatomy, Third Faculty of Medicine, Charles University, Ruska 87, 100 34, Prague, Czech Republic.
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- $a PURPOSE: The soft tissue imaging in micro-CT remains challenging due to its low intrinsic contrast. The aim of this study was to create a simple staining method omitting the usage of contrast agents for ex vivo soft tissue imaging in micro-CT. MATERIALS AND METHODS: Hearts and lungs from 30 mice were used. Twenty-seven organs were either fixed in 97% or 50% ethanol solution or in a series of ascending ethanol concentrations. Images were acquired after 72, 168 and 336 h on a custom-built micro-CT machine and compared to scans of three native samples. RESULTS: Ethanol provided contrast enhancement in all evaluated fixations. Fixation in 97% ethanol resulted in contrast enhancement after 72 h; however, it caused hardening of the samples. Fixation in 50% ethanol provided contrast enhancement after 336 h, with milder hardening, compared to the 97% ethanol fixation, but the visualization of details was worse. The fixation in a series of ascending ethanol concentrations provided the most satisfactory results; all organs were visualized in great detail without tissue damage. CONCLUSIONS: Simple ethanol fixation improves the tissue contrast enhancement in micro-CT. The best results can be obtained with fixation of the soft tissue samples in a series of ascending ethanol concentrations.
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