-
Je něco špatně v tomto záznamu ?
Intragenic Transcriptional cis-Antagonism Across SLC6A3
Y. Zhao, J. Yu, J. Zhao, X. Chen, N. Xiong, T. Wang, H. Qing, Z. Lin,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
DA021409
National Institutes of Health
DA031573
National Institutes of Health
R01 DA021409
NIDA NIH HHS - United States
R21 DA031573
NIDA NIH HHS - United States
NLK
ProQuest Central
od 2019-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2010-02-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2019-01-01 do Před 1 rokem
Psychology Database (ProQuest)
od 2019-01-01 do 2019-12-31
- MeSH
- biologické modely MeSH
- dospělí MeSH
- genetická transkripce * MeSH
- haplotypy genetika MeSH
- lidé MeSH
- minisatelitní repetice genetika MeSH
- myši inbrední C57BL MeSH
- nádorové buněčné linie MeSH
- promotorové oblasti (genetika) * MeSH
- proteiny přenášející dopamin přes plazmatickou membránu genetika MeSH
- transkripční faktory metabolismus MeSH
- zesilovače transkripce MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
A promoter can be regulated by various cis-acting elements so that delineation of the regulatory modes among them may help understand developmental, environmental and genetic mechanisms in gene activity. Here we report that the human dopamine transporter gene SLC6A3 carries a 5' distal 5-kb super enhancer (5KSE) which upregulated the promoter by 5-fold. Interestingly, 5KSE is able to prevent 3' downstream variable number tandem repeats (3'VNTRs) from silencing the promoter. This new enhancer consists of a 5'VNTR and three repetitive sub-elements that are conserved in primates. Two of 5KSE's sub-elements, E-9.7 and E-8.7, upregulate the promoter, but only the later could continue doing so in the presence of 3'VNTRs. Finally, E-8.7 is activated by novel dopaminergic transcription factors including SRP54 and Nfe2l1. Together, these results reveal a multimodal regulatory mechanism in SLC6A3.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19034994
- 003
- CZ-PrNML
- 005
- 20191015104906.0
- 007
- ta
- 008
- 191007s2019 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12035-018-1357-5 $2 doi
- 035 __
- $a (PubMed)30259411
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Zhao, Ying $u Laboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA. School of Pharmacy, Xinxiang Medical University, Xinxiang, 453003, China.
- 245 10
- $a Intragenic Transcriptional cis-Antagonism Across SLC6A3 / $c Y. Zhao, J. Yu, J. Zhao, X. Chen, N. Xiong, T. Wang, H. Qing, Z. Lin,
- 520 9_
- $a A promoter can be regulated by various cis-acting elements so that delineation of the regulatory modes among them may help understand developmental, environmental and genetic mechanisms in gene activity. Here we report that the human dopamine transporter gene SLC6A3 carries a 5' distal 5-kb super enhancer (5KSE) which upregulated the promoter by 5-fold. Interestingly, 5KSE is able to prevent 3' downstream variable number tandem repeats (3'VNTRs) from silencing the promoter. This new enhancer consists of a 5'VNTR and three repetitive sub-elements that are conserved in primates. Two of 5KSE's sub-elements, E-9.7 and E-8.7, upregulate the promoter, but only the later could continue doing so in the presence of 3'VNTRs. Finally, E-8.7 is activated by novel dopaminergic transcription factors including SRP54 and Nfe2l1. Together, these results reveal a multimodal regulatory mechanism in SLC6A3.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a nádorové buněčné linie $7 D045744
- 650 _2
- $a proteiny přenášející dopamin přes plazmatickou membránu $x genetika $7 D050483
- 650 _2
- $a zesilovače transkripce $7 D004742
- 650 _2
- $a haplotypy $x genetika $7 D006239
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a myši inbrední C57BL $7 D008810
- 650 _2
- $a minisatelitní repetice $x genetika $7 D018598
- 650 _2
- $a biologické modely $7 D008954
- 650 12
- $a promotorové oblasti (genetika) $7 D011401
- 650 _2
- $a transkripční faktory $x metabolismus $7 D014157
- 650 12
- $a genetická transkripce $7 D014158
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Yu, Jinlong $u Laboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA.
- 700 1_
- $a Zhao, Juan $u Laboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA. College of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
- 700 1_
- $a Chen, Xiaowu $u Laboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA. Department of Neurology, Shenzhen University General Hospital, Shenzhen, Guangzhou, 518060, China.
- 700 1_
- $a Xiong, Nian $u Laboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA. Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
- 700 1_
- $a Wang, Tao $u Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
- 700 1_
- $a Qing, Hong $u College of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
- 700 1_
- $a Lin, Zhicheng $u Laboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA. zlin@mclean.harvard.edu.
- 773 0_
- $w MED00005280 $t Molecular neurobiology $x 1559-1182 $g Roč. 56, č. 6 (2019), s. 4051-4060
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30259411 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20191007 $b ABA008
- 991 __
- $a 20191015105331 $b ABA008
- 999 __
- $a ok $b bmc $g 1451654 $s 1073544
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 56 $c 6 $d 4051-4060 $e 20180927 $i 1559-1182 $m Molecular neurobiology $n Mol Neurobiol $x MED00005280
- GRA __
- $a DA021409 $p National Institutes of Health
- GRA __
- $a DA031573 $p National Institutes of Health
- GRA __
- $a R01 DA021409 $p NIDA NIH HHS $2 United States
- GRA __
- $a R21 DA031573 $p NIDA NIH HHS $2 United States
- LZP __
- $a Pubmed-20191007
