• Je něco špatně v tomto záznamu ?

Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis

A. Pencík, R. Casanova-Sáez, V. Pilarová, A. Žukauskaite, R. Pinto, JL. Micol, K. Ljung, O. Novák,

. 2018 ; 69 (10) : 2569-2579. [pub] 20180427

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19035364

Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples (<10 mg FW) of Arabidopsis thaliana tissues by in-tip micro solid-phase extraction and fast LC-tandem MS. As a proof of concept, we applied the method to a collection of Arabidopsis mutant lines and identified lines with altered IAA metabolite profiles using multivariate data analysis. Finally, we explored the correlation between IAA metabolite profiles and IAA-related phenotypes. The developed rapid analysis of large numbers of samples (>100 samples d-1) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19035364
003      
CZ-PrNML
005      
20191014105453.0
007      
ta
008      
191007s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/jxb/ery084 $2 doi
035    __
$a (PubMed)29514302
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Pencík, Aleš $u Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden. Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University & Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelů, Olomouc, Czech Republic.
245    10
$a Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis / $c A. Pencík, R. Casanova-Sáez, V. Pilarová, A. Žukauskaite, R. Pinto, JL. Micol, K. Ljung, O. Novák,
520    9_
$a Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples (<10 mg FW) of Arabidopsis thaliana tissues by in-tip micro solid-phase extraction and fast LC-tandem MS. As a proof of concept, we applied the method to a collection of Arabidopsis mutant lines and identified lines with altered IAA metabolite profiles using multivariate data analysis. Finally, we explored the correlation between IAA metabolite profiles and IAA-related phenotypes. The developed rapid analysis of large numbers of samples (>100 samples d-1) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes.
650    _2
$a Arabidopsis $x genetika $x metabolismus $7 D017360
650    _2
$a chromatografie kapalinová $7 D002853
650    _2
$a vysoce účinné nukleotidové sekvenování $x metody $7 D059014
650    _2
$a kyseliny indoloctové $x metabolismus $7 D007210
650    _2
$a multivariační analýza $7 D015999
650    12
$a mutace $7 D009154
650    _2
$a rostlinné proteiny $x analýza $7 D010940
650    _2
$a extrakce na pevné fázi $7 D052616
650    _2
$a tandemová hmotnostní spektrometrie $7 D053719
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Casanova-Sáez, Rubén $u Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
700    1_
$a Pilarová, Veronika $u Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského, Hradec Králové, Czech Republic.
700    1_
$a Žukauskaite, Asta $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University & Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelu, Olomouc, Czech Republic.
700    1_
$a Pinto, Rui $u Computational Life Science Cluster (CLiC), Chemistry department (KBC), Umeå University, Umeå, Sweden.
700    1_
$a Micol, José Luis $u Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, Elche, Alicante, Spain.
700    1_
$a Ljung, Karin $u Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
700    1_
$a Novák, Ondrej $u Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden. Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University & Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelů, Olomouc, Czech Republic.
773    0_
$w MED00006559 $t Journal of experimental botany $x 1460-2431 $g Roč. 69, č. 10 (2018), s. 2569-2579
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29514302 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191014105917 $b ABA008
999    __
$a ok $b bmc $g 1452024 $s 1073914
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 69 $c 10 $d 2569-2579 $e 20180427 $i 1460-2431 $m Journal of Experimental Botany $n J Exp Bot $x MED00006559
LZP    __
$a Pubmed-20191007
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace