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Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol-proton symporters
J. Zemancíková, M. Dušková, H. Elicharová, K. Papoušková, H. Sychrová,
Language English Country England, Great Britain
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2015-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2015-01-01 to 1 year ago
Public Health Database (ProQuest)
from 2015-01-01 to 1 year ago
PubMed
29385575
DOI
10.1093/femsle/fny020
Knihovny.cz E-resources
- MeSH
- Dekkera genetics isolation & purification metabolism physiology MeSH
- Species Specificity MeSH
- Fungal Proteins genetics metabolism MeSH
- Genome, Bacterial genetics MeSH
- Glycerol metabolism MeSH
- Osmoregulation genetics MeSH
- Protons MeSH
- Recombinant Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae genetics MeSH
- Symporters genetics metabolism MeSH
- Genetic Complementation Test MeSH
- Wine microbiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
Dekkera bruxellensis is important for lambic beer fermentation but is considered a spoilage yeast in wine fermentation. We compared two D. bruxellensis strains isolated from wine and found that they differ in some basic properties, including osmotolerance. The genomes of both strains contain two highly similar copies of genes encoding putative glycerol-proton symporters from the STL family that are important for yeast osmotolerance. Cloning of the two DbSTL genes and their expression in suitable osmosensitive Saccharomyces cerevisiae mutants revealed that both identified genes encode functional glycerol uptake systems, but only DbStl2 has the capacity to improve the osmotolerance of S. cerevisiae cells.
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