Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment

MR. Zarone, G. Misso, A. Grimaldi, S. Zappavigna, M. Russo, E. Amler, MT. Di Martino, N. Amodio, P. Tagliaferri, P. Tassone, M. Caraglia,

. 2017 ; 7 (1) : 17949. [pub] 20171220

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19035468

MiR-34a acts as tumor suppressor microRNA (miRNA) in several cancers, including multiple myeloma (MM), by controlling the expression of target proteins involved in cell cycle, differentiation and apoptosis. Here, we have investigated the combination between miR-34a and γ-secretase inhibitor (γSI), Sirtinol or zoledronic acid (ZOL) in order to enhance the inhibitory action of this miRNA on its canonical targets such as Notch1 and SIRT1, and on Ras/MAPK-dependent pathways. Our data demonstrate that miR-34a synthetic mimics significantly enhance the anti-tumor activity of all the above-mentioned anti-cancer agents in RPMI 8226 MM cells. We found that γSI enhanced miR-34a-dependent anti-tumor effects by activating the extrinsic apoptotic pathway which could overcome the cytoprotective autophagic mechanism. Moreover, the combination between miR-34a and γSI increased the cell surface calreticulin (CRT) expression, that is well known for triggering anti-tumor immunological response. The combination between miR-34a and Sirtinol induced the activation of an intrinsic apoptotic pathway along with increased surface expression of CRT. Regarding ZOL, we found a powerful growth inhibition after enforced miR-34a expression, which was not likely attributable to neither apoptosis nor autophagy modulation. Based on our data, the combination of miR-34a with other anti-cancer agents appears a promising anti-MM strategy deserving further investigation.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19035468
003      
CZ-PrNML
005      
20191008113032.0
007      
ta
008      
191007s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-017-18186-0 $2 doi
035    __
$a (PubMed)29263373
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Zarone, Mayra Rachele $u Department of Biochemistry, Biophysics and General Pathology, University of Campania "L. Vanvitelli", Naples, Italy.
245    10
$a Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment / $c MR. Zarone, G. Misso, A. Grimaldi, S. Zappavigna, M. Russo, E. Amler, MT. Di Martino, N. Amodio, P. Tagliaferri, P. Tassone, M. Caraglia,
520    9_
$a MiR-34a acts as tumor suppressor microRNA (miRNA) in several cancers, including multiple myeloma (MM), by controlling the expression of target proteins involved in cell cycle, differentiation and apoptosis. Here, we have investigated the combination between miR-34a and γ-secretase inhibitor (γSI), Sirtinol or zoledronic acid (ZOL) in order to enhance the inhibitory action of this miRNA on its canonical targets such as Notch1 and SIRT1, and on Ras/MAPK-dependent pathways. Our data demonstrate that miR-34a synthetic mimics significantly enhance the anti-tumor activity of all the above-mentioned anti-cancer agents in RPMI 8226 MM cells. We found that γSI enhanced miR-34a-dependent anti-tumor effects by activating the extrinsic apoptotic pathway which could overcome the cytoprotective autophagic mechanism. Moreover, the combination between miR-34a and γSI increased the cell surface calreticulin (CRT) expression, that is well known for triggering anti-tumor immunological response. The combination between miR-34a and Sirtinol induced the activation of an intrinsic apoptotic pathway along with increased surface expression of CRT. Regarding ZOL, we found a powerful growth inhibition after enforced miR-34a expression, which was not likely attributable to neither apoptosis nor autophagy modulation. Based on our data, the combination of miR-34a with other anti-cancer agents appears a promising anti-MM strategy deserving further investigation.
650    _2
$a protinádorové látky $x terapeutické užití $7 D000970
650    _2
$a apoptóza $x účinky léků $7 D017209
650    _2
$a benzamidy $x terapeutické užití $7 D001549
650    _2
$a buněčný cyklus $x účinky léků $7 D002453
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a lidé $7 D006801
650    _2
$a mikro RNA $x terapeutické užití $7 D035683
650    _2
$a mnohočetný myelom $x farmakoterapie $7 D009101
650    _2
$a naftoly $x terapeutické užití $7 D009284
650    _2
$a kyselina zoledronová $x terapeutické užití $7 D000077211
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Misso, Gabriella $u Department of Biochemistry, Biophysics and General Pathology, University of Campania "L. Vanvitelli", Naples, Italy.
700    1_
$a Grimaldi, Anna $u Department of Biochemistry, Biophysics and General Pathology, University of Campania "L. Vanvitelli", Naples, Italy.
700    1_
$a Zappavigna, Silvia $u Department of Biochemistry, Biophysics and General Pathology, University of Campania "L. Vanvitelli", Naples, Italy.
700    1_
$a Russo, Margherita $u Department of Biochemistry, Biophysics and General Pathology, University of Campania "L. Vanvitelli", Naples, Italy.
700    1_
$a Amler, Evzen $u Institute of Biophysics, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Di Martino, Maria Teresa $u Department of Experimental and Clinical Medicine, Magna Graecia University, Salvatore Venuta University Campus, Catanzaro, Italy.
700    1_
$a Amodio, Nicola $u Department of Experimental and Clinical Medicine, Magna Graecia University, Salvatore Venuta University Campus, Catanzaro, Italy.
700    1_
$a Tagliaferri, Pierosandro $u Department of Experimental and Clinical Medicine, Magna Graecia University, Salvatore Venuta University Campus, Catanzaro, Italy.
700    1_
$a Tassone, Pierfrancesco $u Department of Experimental and Clinical Medicine, Magna Graecia University, Salvatore Venuta University Campus, Catanzaro, Italy. Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania, USA.
700    1_
$a Caraglia, Michele $u Department of Biochemistry, Biophysics and General Pathology, University of Campania "L. Vanvitelli", Naples, Italy. Michele.caraglia@unicampania.it. Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania, USA. Michele.caraglia@unicampania.it.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 7, č. 1 (2017), s. 17949
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29263373 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191008113448 $b ABA008
999    __
$a ok $b bmc $g 1452128 $s 1074018
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 7 $c 1 $d 17949 $e 20171220 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20191007

Find record

Citation metrics

Archiving options