Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

New Design of the Electrophoretic Part of CLARITY Technology for Confocal Light Microscopy of Rat and Human Brains

P. Zach, J. Mrzílková, J. Pala, L. Uttl, V. Kútna, V. Musil, B. Sommerová, P. Tůma,

. 2019 ; 9 (9) : . [pub] 20190829

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19035743

Grantová podpora
Q35, Q41, P31 and 260388/SVV/2019 Grantová Agentura, Univerzita Karlova
18-04902S and GBP304/12/G069 Grantová Agentura České Republiky

BACKGROUND: CLARITY is a method of rendering postmortem brain tissue transparent using acrylamide-based hydrogels so that this tissue could be further used for immunohistochemistry, molecular biology, or gross anatomical studies. Published papers using the CLARITY method have included studies on human brains suffering from Alzheimer's disease using mouse spinal cords as animal models for multiple sclerosis. METHODS: We modified the original design of the Chung CLARITY system by altering the electrophoretic flow-through cell, the shape of the platinum electrophoresis electrodes and their positions, as well as the cooling and recirculation system, so that it provided a greater effect and can be used in any laboratory. RESULTS: The adapted CLARITY system is assembled from basic laboratory components, in contrast to the original design. The modified CLARITY system was tested both on rat brain stained with a rabbit polyclonal anti-Iba-1 for microglial cells and on human nucleus accumbens stained with parvalbumin and tyrosine hydroxylase for visualization of specific neurons by confocal laser scanning microscopy. CONCLUSIONS: Our design has the advantage of simplicity, functional robustness, and minimal requirement for specialized additional items for the construction of the CLARITY apparatus.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19035743
003      
CZ-PrNML
005      
20201102104348.0
007      
ta
008      
191007s2019 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/brainsci9090218 $2 doi
035    __
$a (PubMed)31470513
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Zach, Petr $u Department of Anatomy, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic. National Institute of Mental Health, Topolová 748, 250 67 Klecany, Czech Republic.
245    10
$a New Design of the Electrophoretic Part of CLARITY Technology for Confocal Light Microscopy of Rat and Human Brains / $c P. Zach, J. Mrzílková, J. Pala, L. Uttl, V. Kútna, V. Musil, B. Sommerová, P. Tůma,
520    9_
$a BACKGROUND: CLARITY is a method of rendering postmortem brain tissue transparent using acrylamide-based hydrogels so that this tissue could be further used for immunohistochemistry, molecular biology, or gross anatomical studies. Published papers using the CLARITY method have included studies on human brains suffering from Alzheimer's disease using mouse spinal cords as animal models for multiple sclerosis. METHODS: We modified the original design of the Chung CLARITY system by altering the electrophoretic flow-through cell, the shape of the platinum electrophoresis electrodes and their positions, as well as the cooling and recirculation system, so that it provided a greater effect and can be used in any laboratory. RESULTS: The adapted CLARITY system is assembled from basic laboratory components, in contrast to the original design. The modified CLARITY system was tested both on rat brain stained with a rabbit polyclonal anti-Iba-1 for microglial cells and on human nucleus accumbens stained with parvalbumin and tyrosine hydroxylase for visualization of specific neurons by confocal laser scanning microscopy. CONCLUSIONS: Our design has the advantage of simplicity, functional robustness, and minimal requirement for specialized additional items for the construction of the CLARITY apparatus.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Mrzílková, Jana $u Department of Anatomy, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.
700    1_
$a Pala, Jan $u National Institute of Mental Health, Topolová 748, 250 67 Klecany, Czech Republic. Department of Pathophysiology, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.
700    1_
$a Uttl, Libor $u National Institute of Mental Health, Topolová 748, 250 67 Klecany, Czech Republic.
700    1_
$a Kútna, Viera $u National Institute of Mental Health, Topolová 748, 250 67 Klecany, Czech Republic.
700    1_
$a Musil, Vladimír, $d 1976- $u Centre of Scientific Information, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic. $7 xx0096743
700    1_
$a Sommerová, Blanka $u Department of Hygiene, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.
700    1_
$a Tůma, Petr $u Department of Hygiene, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic. petr.tuma@lf3.cuni.cz.
773    0_
$w MED00198694 $t Brain sciences $x 2076-3425 $g Roč. 9, č. 9 (2019)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31470513 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20201102104346 $b ABA008
999    __
$a ind $b bmc $g 1452403 $s 1074293
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 9 $c 9 $e 20190829 $i 2076-3425 $m Brain sciences $n Brain Sci $x MED00198694
GRA    __
$a Q35, Q41, P31 and 260388/SVV/2019 $p Grantová Agentura, Univerzita Karlova
GRA    __
$a 18-04902S and GBP304/12/G069 $p Grantová Agentura České Republiky
LZP    __
$a Pubmed-20191007

Najít záznam

Citační ukazatele

Možnosti archivace