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Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

M. Buzgo, E. Filova, AM. Staffa, M. Rampichova, M. Doupnik, K. Vocetkova, V. Lukasova, R. Kolcun, D. Lukas, A. Necas, E. Amler,

. 2018 ; 12 (3) : 583-597. [pub] 20171129

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19045612

Grant support
NV15-33094A MZ0 CEP Register
NV16-29680A MZ0 CEP Register
NV16-29680A MZ0 CEP Register

In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.

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$a Buzgo, Matej $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. University Centre of Energetically Efficient Buildings, Czech Technical University, Buštěhrad, Czech Republic.
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$a In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.
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$a Filova, Eva $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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$a Staffa, Andrea Mickova $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. University Centre of Energetically Efficient Buildings, Czech Technical University, Buštěhrad, Czech Republic.
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$a Rampichova, Michala $u Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. University Centre of Energetically Efficient Buildings, Czech Technical University, Buštěhrad, Czech Republic.
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$a Doupnik, Miroslav $u University Centre of Energetically Efficient Buildings, Czech Technical University, Buštěhrad, Czech Republic.
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$a Vocetkova, Karolina $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. University Centre of Energetically Efficient Buildings, Czech Technical University, Buštěhrad, Czech Republic.
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$a Kolcun, Radka $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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$a Necas, Alois $u Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
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$a Amler, Evzen $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. University Centre of Energetically Efficient Buildings, Czech Technical University, Buštěhrad, Czech Republic.
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