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Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics
J. Lačná, P. Ďurč, M. Greguš, J. Skřičková, M. Doubková, E. Pokojová, D. Kindlová, J. Dolina, Š. Konečný, F. Foret, P. Kubáň,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Grantová podpora
NV17-31945A
MZ0
CEP - Centrální evidence projektů
- MeSH
- butyráty analýza MeSH
- dechové testy metody MeSH
- dospělí MeSH
- elektroforéza kapilární metody MeSH
- gastroezofageální reflux diagnóza metabolismus MeSH
- ionty analýza metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- lidé středního věku MeSH
- lidé MeSH
- propionáty analýza MeSH
- senioři MeSH
- sodík analýza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
In this study, the ionic profile and pH of exhaled breath condensate (EBC) in a group of patients with acid and weakly acid reflux and no-reflux controls were compared. A portable sampler was used for non-invasive EBC collection from five exhalations. The ionic profile (anions, cations, organic acids) and pH of the collected EBC samples were measured by capillary electrophoresis with contactless conductivity detection and a pH microelectrode, respectively. Several ions were elevated in the patient groups. Sodium cation was elevated in weakly acid reflux (significance level p < 0.01) and acid reflux (p < 0.05) compared to no-reflux controls. Butyrate and propionate were elevated in both acid reflux and weakly acid reflux compared to no-reflux controls (butyrate: p < 0.01, propionate: p < 0.05). The median values of pH (after de-aeration with N2) were also significantly higher (p < 0.01) in groups with acid reflux and weakly acid reflux than in the control group with no reflux. The ionic analysis and simultaneous pH measurement offer a simple, cheap, fast, and non-invasive approach in gastroesophageal reflux disease diagnostics.
Citace poskytuje Crossref.org
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- $a Lačná, Júlia $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, Brno 602 00, Czech Republic; Department of Chemistry, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
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- $a In this study, the ionic profile and pH of exhaled breath condensate (EBC) in a group of patients with acid and weakly acid reflux and no-reflux controls were compared. A portable sampler was used for non-invasive EBC collection from five exhalations. The ionic profile (anions, cations, organic acids) and pH of the collected EBC samples were measured by capillary electrophoresis with contactless conductivity detection and a pH microelectrode, respectively. Several ions were elevated in the patient groups. Sodium cation was elevated in weakly acid reflux (significance level p < 0.01) and acid reflux (p < 0.05) compared to no-reflux controls. Butyrate and propionate were elevated in both acid reflux and weakly acid reflux compared to no-reflux controls (butyrate: p < 0.01, propionate: p < 0.05). The median values of pH (after de-aeration with N2) were also significantly higher (p < 0.01) in groups with acid reflux and weakly acid reflux than in the control group with no reflux. The ionic analysis and simultaneous pH measurement offer a simple, cheap, fast, and non-invasive approach in gastroesophageal reflux disease diagnostics.
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- $a Ďurč, Pavol $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, Brno 602 00, Czech Republic; Department of Chemistry, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
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