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No magnesium is needed for binding of the stimulator of interferon genes to cyclic dinucleotides
M. Smola, G. Birkus, E. Boura,
Language English Country United States
Document type Journal Article
Grant support
RVO:61388963
Akademie Věd České Republiky
NLK
Free Medical Journals
from 2014 to 2 years ago
PubMed Central
from 2014 to 1 year ago
Europe PubMed Central
from 2014 to 2 years ago
- MeSH
- Cyclic GMP chemistry metabolism MeSH
- Magnesium metabolism MeSH
- Crystallography, X-Ray MeSH
- Membrane Proteins chemistry genetics metabolism MeSH
- Publication type
- Journal Article MeSH
Stimulator of interferon genes (STING) binds cyclic dinucleotides (CDNs), which induce a large conformational change of the protein. The structural basis of activation of STING by CDNs is rather well understood. Unliganded STING forms an open dimer that undergoes a large conformational change (∼10 Å) to a closed conformation upon the binding of a CDN molecule. This event activates downstream effectors of STING and subsequently leads to activation of the type 1 interferon response. However, a previously solved structure of STING with 3',3'-c-di-GMP shows Mg atoms mediating the interaction of STING with this CDN. Here, it is shown that no Mg atoms are needed for this interaction; in fact, magnesium can in some cases obstruct the binding of a CDN to STING.
References provided by Crossref.org
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- $a Stimulator of interferon genes (STING) binds cyclic dinucleotides (CDNs), which induce a large conformational change of the protein. The structural basis of activation of STING by CDNs is rather well understood. Unliganded STING forms an open dimer that undergoes a large conformational change (∼10 Å) to a closed conformation upon the binding of a CDN molecule. This event activates downstream effectors of STING and subsequently leads to activation of the type 1 interferon response. However, a previously solved structure of STING with 3',3'-c-di-GMP shows Mg atoms mediating the interaction of STING with this CDN. Here, it is shown that no Mg atoms are needed for this interaction; in fact, magnesium can in some cases obstruct the binding of a CDN to STING.
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