Plasma membrane (PM) lipid composition and domain organization are modulated by polarized exocytosis. Conversely, targeting of secretory vesicles at specific domains in the PM is carried out by exocyst complexes, which contain EXO70 subunits that play a significant role in the final recognition of the target membrane. As we have shown previously, a mature Arabidopsis trichome contains a basal domain with a thin cell wall and an apical domain with a thick secondary cell wall, which is developed in an EXO70H4-dependent manner. These domains are separated by a cell wall structure named the Ortmannian ring. Using phospholipid markers, we demonstrate that there are two distinct PM domains corresponding to these cell wall domains. The apical domain is enriched in phosphatidic acid (PA) and phosphatidylserine, with an undetectable amount of phosphatidylinositol 4,5-bisphosphate (PIP2), whereas the basal domain is PIP2-rich. While the apical domain recruits EXO70H4, the basal domain recruits EXO70A1, which corresponds to the lipid-binding capacities of these two paralogs. Loss of EXO70H4 results in a loss of the Ortmannian ring border and decreased apical PA accumulation, which causes the PA and PIP2 domains to merge together. Using transmission electron microscopy, we describe these accumulations as a unique anatomical feature of the apical cell wall-radially distributed rod-shaped membranous pockets, where both EXO70H4 and lipid markers are immobilized.

Department of Experimental Plant Biology Faculty of Science Charles University 12800 Prague Czech Republic

Department of Experimental Plant Biology Faculty of Science Charles University 12800 Prague Czech Republic Institute of Experimental Botany Czech Academy of Sciences 165 02 Prague Czech Republic

Institute of Experimental Botany Czech Academy of Sciences 165 02 Prague Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc20006069

003      

CZ-PrNML

005      

20200518132317.0

007      

ta

008      

200511s2019 sz f 000 0|eng||

009      

AR

024    7_

$a 10.3390/ijms20153803 $2 doi

035    __

$a (PubMed)31382643

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Kubátová, Zdeňka $u Department of Experimental Plant Biology, Faculty of Science, Charles University, 12800 Prague, Czech Republic.

245    10

$a Arabidopsis Trichome Contains Two Plasma Membrane Domains with Different Lipid Compositions Which Attract Distinct EXO70 Subunits / $c Z. Kubátová, P. Pejchar, M. Potocký, J. Sekereš, V. Žárský, I. Kulich,

520    9_

$a Plasma membrane (PM) lipid composition and domain organization are modulated by polarized exocytosis. Conversely, targeting of secretory vesicles at specific domains in the PM is carried out by exocyst complexes, which contain EXO70 subunits that play a significant role in the final recognition of the target membrane. As we have shown previously, a mature Arabidopsis trichome contains a basal domain with a thin cell wall and an apical domain with a thick secondary cell wall, which is developed in an EXO70H4-dependent manner. These domains are separated by a cell wall structure named the Ortmannian ring. Using phospholipid markers, we demonstrate that there are two distinct PM domains corresponding to these cell wall domains. The apical domain is enriched in phosphatidic acid (PA) and phosphatidylserine, with an undetectable amount of phosphatidylinositol 4,5-bisphosphate (PIP2), whereas the basal domain is PIP2-rich. While the apical domain recruits EXO70H4, the basal domain recruits EXO70A1, which corresponds to the lipid-binding capacities of these two paralogs. Loss of EXO70H4 results in a loss of the Ortmannian ring border and decreased apical PA accumulation, which causes the PA and PIP2 domains to merge together. Using transmission electron microscopy, we describe these accumulations as a unique anatomical feature of the apical cell wall-radially distributed rod-shaped membranous pockets, where both EXO70H4 and lipid markers are immobilized.

650    _2

$a Arabidopsis $x chemie $x genetika $7 D017360

650    _2

$a proteiny huseníčku $x chemie $x genetika $7 D029681

650    _2

$a buněčná membrána $x chemie $x genetika $7 D002462

650    _2

$a exocytóza $x genetika $7 D005089

650    _2

$a membránové lipidy $x genetika $x metabolismus $7 D008563

650    _2

$a fosfatidylinositol-4,5-difosfát $x chemie $x metabolismus $7 D019269

650    _2

$a fosfatidylseriny $x chemie $x genetika $7 D010718

650    _2

$a trichomy $x chemie $x genetika $7 D064201

650    _2

$a vezikulární transportní proteiny $x chemie $x genetika $7 D033921

655    _2

$a časopisecké články $7 D016428

700    1_

$a Pejchar, Přemysl $u Department of Experimental Plant Biology, Faculty of Science, Charles University, 12800 Prague, Czech Republic. Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech Republic.

700    1_

$a Potocký, Martin $u Department of Experimental Plant Biology, Faculty of Science, Charles University, 12800 Prague, Czech Republic. Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech Republic.

700    1_

$a Sekereš, Juraj $u Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech Republic.

700    1_

$a Žárský, Viktor $u Department of Experimental Plant Biology, Faculty of Science, Charles University, 12800 Prague, Czech Republic. Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech Republic.

700    1_

$a Kulich, Ivan $u Department of Experimental Plant Biology, Faculty of Science, Charles University, 12800 Prague, Czech Republic. kulich@natur.cuni.cz.

773    0_

$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 20, č. 15 (2019)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/31382643 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20200511 $b ABA008

991    __

$a 20200518132317 $b ABA008

999    __

$a ok $b bmc $g 1524927 $s 1096125

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2019 $b 20 $c 15 $e 20190803 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142

GRA    __

$a 18-12579S $p Grantová Agentura České Republiky

GRA    __

$a NPUI LO1417 $p Ministerstvo Školství, Mládeže a Tělovýchovy

GRA    __

$a CZ.1.05/4.1.00/16.0347 $p European Regional Development Fund

LZP    __

$a Pubmed-20200511