The West Nile virus (WNV) is a mosquito-borne flavivirus causing meningoencephalitis in humans and animals. Due to their particular susceptibility to WNV infection, horses serve as a sentinel species. In a population of Romanian semi-feral horses living in the Danube delta region, we have analyzed the distribution of candidate polymorphic genetic markers between anti WNV-IgG seropositive and seronegative horses. Thirty-six SNPs located in 28 immunity-related genes and 26 microsatellites located in the MHC and LY49 complex genomic regions were genotyped in 57 seropositive and 32 seronegative horses. The most significant association (pcorr < 0.0002) was found for genotypes composed of markers of the SLC11A1 and TLR4 genes. Markers of five other candidate genes (ADAM17, CXCR3, IL12A, MAVS, TNFA), along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status in this model horse population. The OAS1 gene, previously associated with WNV-induced clinical disease, was not associated with the presence of anti-WNV antibodies.

CEITEC VFU University of Veterinary and Pharmaceutical Sciences Palackeho 1 61242 Brno Czech Republic Department of Pathology and Parasitology University of Veterinary and Pharmaceutical Sciences Palackeho tr 1 612 42 Brno Czech Republic Institute of Parasitology Biology Centre of the Czech Academy of Sciences Branišovská 31 České Budějovice 370 05 Czech Republic

Department of Animal Genetics Faculty of Veterinary Medicine University of Veterinary and Pharmaceutical Sciences Palackeho 1 61242 Brno Czech Republic

Department of Animal Genetics Faculty of Veterinary Medicine University of Veterinary and Pharmaceutical Sciences Palackeho 1 61242 Brno Czech Republic CEITEC VFU University of Veterinary and Pharmaceutical Sciences Palackeho 1 61242 Brno Czech Republic

Department of Animal Morphology Physiology and Genetics Faculty of Agronomy Mendel University in Brno Zemědělská 1 1665 613 00 Brno Czech Republic CEITEC MENDELU Mendel University in Brno Zemědělská 1 1665 613 00 Brno Czech Republic

Department of Parasitology and Parasitic Diseases University of Agricultural Sciences and Veterinary Medicine Cluj Napoca Calea Mănăştur 3 5 400362 Cluj Napoca Romania

Department of Pathology and Parasitology University of Veterinary and Pharmaceutical Sciences Palackeho tr 1 612 42 Brno Czech Republic

Faculty of Medicine Institute of Biostatistics and Analyses Masaryk University Kamenice 753 5 625 00 Brno Czech Republic

Institute of Vertebrate Biology of the Academy of Sciences Květná 8 60365 Brno Czech Republic

SVU Jihlava Rantirovska 93 20 Horni Kosov 58601 Jihlava Czech Republic

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$a Stejskalova, K $u Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic.

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$a Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta / $c K. Stejskalova, E. Janova, C. Horecky, E. Horecka, P. Vaclavek, Z. Hubalek, K. Relling, M. Cvanova, G. D'Amico, AD. Mihalca, D. Modry, A. Knoll, P. Horin,

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$a The West Nile virus (WNV) is a mosquito-borne flavivirus causing meningoencephalitis in humans and animals. Due to their particular susceptibility to WNV infection, horses serve as a sentinel species. In a population of Romanian semi-feral horses living in the Danube delta region, we have analyzed the distribution of candidate polymorphic genetic markers between anti WNV-IgG seropositive and seronegative horses. Thirty-six SNPs located in 28 immunity-related genes and 26 microsatellites located in the MHC and LY49 complex genomic regions were genotyped in 57 seropositive and 32 seronegative horses. The most significant association (pcorr < 0.0002) was found for genotypes composed of markers of the SLC11A1 and TLR4 genes. Markers of five other candidate genes (ADAM17, CXCR3, IL12A, MAVS, TNFA), along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status in this model horse population. The OAS1 gene, previously associated with WNV-induced clinical disease, was not associated with the presence of anti-WNV antibodies.

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$a Janova, E $u Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic. CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic.

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$a Horecky, C $u Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic. CEITEC-MENDELU, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.

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$a Horecka, E $u Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic. CEITEC-MENDELU, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.

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$a Vaclavek, P $u SVU Jihlava, Rantirovska 93/20, Horni Kosov, 58601, Jihlava, Czech Republic.

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$a Hubalek, Z $u Institute of Vertebrate Biology of the Academy of Sciences, Květná 8, 60365, Brno, Czech Republic.

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$a Relling, K $u Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1, 612 42, Brno, Czech Republic.

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$a Cvanova, M $u Faculty of Medicine, Institute of Biostatistics and Analyses, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.

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$a D'Amico, G $u Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400362, Cluj-Napoca, Romania.

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$a Mihalca, A D $u Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400362, Cluj-Napoca, Romania.

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$a Modry, D $u CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic. Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1, 612 42, Brno, Czech Republic. Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice, 370 05, Czech Republic.

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$a Knoll, A $u Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic. CEITEC-MENDELU, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.

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$a Horin, P $u Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic. horin@dior.ics.muni.cz. CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic. horin@dior.ics.muni.cz.

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