The West Nile virus (WNV) is a mosquito-borne flavivirus causing meningoencephalitis in humans and animals. Due to their particular susceptibility to WNV infection, horses serve as a sentinel species. In a population of Romanian semi-feral horses living in the Danube delta region, we have analyzed the distribution of candidate polymorphic genetic markers between anti WNV-IgG seropositive and seronegative horses. Thirty-six SNPs located in 28 immunity-related genes and 26 microsatellites located in the MHC and LY49 complex genomic regions were genotyped in 57 seropositive and 32 seronegative horses. The most significant association (pcorr < 0.0002) was found for genotypes composed of markers of the SLC11A1 and TLR4 genes. Markers of five other candidate genes (ADAM17, CXCR3, IL12A, MAVS, TNFA), along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status in this model horse population. The OAS1 gene, previously associated with WNV-induced clinical disease, was not associated with the presence of anti-WNV antibodies.
- MeSH
- ELISA MeSH
- jednonukleotidový polymorfismus genetika MeSH
- koně krev genetika imunologie MeSH
- mikrosatelitní repetice genetika MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- protilátky virové analýza krev MeSH
- sentinelové organismy MeSH
- virus západního Nilu patogenita MeSH
- západonilská horečka genetika imunologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Rumunsko MeSH
- MeSH
- biologické modely MeSH
- kolifágy genetika izolace a purifikace fyziologie MeSH
- koně krev virologie MeSH
- krev virologie MeSH
- střeva virologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This research was carried out on five clinically healthy Sella Italiana horses to determine the daily rhythm of total proteins and their fractions, to establish if these rhythms are endogenously generated and to assess the role of light as synchroniser of these rhythms. Blood samples were collected from each subject every 3 h over a period of 48 h, starting at 9:00 on day 1 and finishing at 9:00 on day 3, into vacutainer tubes without an anticoagulant via intravenous cannulas inserted into the jugular vein. Total serum proteins, albumin, α1-, α2-, β1-, β2- and γ-globulin concentrations were assessed in all samples. The application of two-way ANOVA showed a significant effect of the time of day on total proteins, albumin, β1- and β2-globulins, and of the experimental conditions on total proteins α1-, α2-, β1-, β2- and γ-globulins. No statistical modifications were observed on the A/G ratio. Daily rhythmicity was exhibited only by total proteins and albumin during the L/D cycle. We can claim that the fluctuation of serum total proteins and albumin concentrations are daily and not circadian and that they are driven by the L/D cycle.
- MeSH
- cirkadiánní rytmus fyziologie MeSH
- elektroforéza sérových bílkovin statistika a číselné údaje MeSH
- fotoperioda MeSH
- koně fyziologie krev MeSH
- krevní proteiny fyziologie izolace a purifikace MeSH
- periodicita MeSH
- sérové globuliny fyziologie izolace a purifikace MeSH
- sérový albumin fyziologie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- MeSH
- ektroforéza na škrobovém gelu metody využití MeSH
- elektroforéza sérových bílkovin * metody využití MeSH
- experimenty na lidech MeSH
- gama-globuliny analýza imunologie izolace a purifikace MeSH
- hematologické nádory * enzymologie imunologie krev MeSH
- koně imunologie krev MeSH
- králíci imunologie krev MeSH
- lidé MeSH
- precipitinové testy metody využití MeSH
- sérové globuliny * analýza imunologie izolace a purifikace MeSH
- sérum enzymologie MeSH
- statistika jako téma MeSH
- Check Tag
- králíci imunologie krev MeSH
- lidé MeSH
- MeSH
- aglutinace * genetika imunologie účinky léků MeSH
- biomedicínský výzkum metody MeSH
- dobytek krev MeSH
- koně krev MeSH
- krevní buňky * metabolismus účinky léků MeSH
- lidé krev MeSH
- ovce krev MeSH
- prasata krev MeSH
- rostlinné extrakty krev metabolismus MeSH
- rostlinné lektiny * izolace a purifikace krev MeSH
- statistika jako téma MeSH
- zvířata MeSH
- Check Tag
- lidé krev MeSH
- zvířata MeSH
