The West Nile virus (WNV) is a mosquito-borne flavivirus causing meningoencephalitis in humans and animals. Due to their particular susceptibility to WNV infection, horses serve as a sentinel species. In a population of Romanian semi-feral horses living in the Danube delta region, we have analyzed the distribution of candidate polymorphic genetic markers between anti WNV-IgG seropositive and seronegative horses. Thirty-six SNPs located in 28 immunity-related genes and 26 microsatellites located in the MHC and LY49 complex genomic regions were genotyped in 57 seropositive and 32 seronegative horses. The most significant association (pcorr < 0.0002) was found for genotypes composed of markers of the SLC11A1 and TLR4 genes. Markers of five other candidate genes (ADAM17, CXCR3, IL12A, MAVS, TNFA), along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status in this model horse population. The OAS1 gene, previously associated with WNV-induced clinical disease, was not associated with the presence of anti-WNV antibodies.
- MeSH
- ELISA MeSH
- jednonukleotidový polymorfismus genetika MeSH
- koně krev genetika imunologie MeSH
- mikrosatelitní repetice genetika MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- protilátky virové analýza krev MeSH
- sentinelové organismy MeSH
- virus západního Nilu patogenita MeSH
- západonilská horečka genetika imunologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Rumunsko MeSH
Rheumatoid factor (RF) is used in human and veterinary medicine in the form of IgM RF traditionally to support the diagnosis of rheumatoid arthritis (RA). In the latest diagnostic criteria, presence of anti - citrullinated protein antibodies (ACPA) was added to the grading system for the diagnosis of RA in humans. A change which is not integrated or routinely used in veterinary medicine. The criteria changed partly because of RF's diagnostic shortcomings, which include its increased titer detection in humans with non-rheumatoid diseases, inability to predict the disease and increased titers over the limit in the older population. Clinical signs similar to human RA were reported in horses in a condition known as idiopathic polysynovitis. Similarities in the clinical presentation to RA raised a question to the usability of RF and ACPA in horses. In our study, sixty clinically healthy horses, ranging from 3 days to 30 years of age, were evaluated for their serum levels of IgM RF. 55 of these horses were tested for ACPA, using methods of ELISA measuring Anti - CFG (Anti citrullinated fibrinogen antibody). The results of the study demonstrated the existence of an age-dependent increase in the level of IgM RF up to the age of about 9 years and ACPA's independence of the horse's age as well as both markers independence of the horse sex.
- MeSH
- autoprotilátky krev MeSH
- biologické markery krev MeSH
- ELISA MeSH
- imunoglobulin G krev MeSH
- imunoglobulin M krev MeSH
- koně imunologie MeSH
- protilátky proti citrulinovaným peptidům krev MeSH
- revmatoidní artritida MeSH
- revmatoidní faktor krev MeSH
- synovitida diagnóza imunologie veterinární MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Insect bite hypersensitivity (IBH) is an allergic dermatitis of horses caused by bites of insects. IBH is a multifactorial disease with contribution of genetic and environmental factors. Candidate gene association analysis of IBH was performed in a group of 89 Icelandic horses all born in Iceland and imported to Europe. Horses were classified in IBH-affected and non-affected based on clinical signs and history of recurrent dermatitis, and on the results of an in vitro sulfidoleukotriene (sLT)-release assay with Culicoides nubeculosus and Simulium vittatum extract. Different genetic markers were tested for association with IBH by the Fisher's exact test. The effect of the major histocompatibility complex (MHC) gene region was studied by genotyping five microsatellites spanning the MHC region (COR112, COR113, COR114, UM011 and UMN-JH34-2), and exon 2 polymorphisms of the class II Eqca-DRA gene. Associations with Eqca-DRA and COR113 were identified (p < 0.05). In addition, a panel of 20 single nucleotide polymorphisms (SNPs) in 17 candidate allergy-related genes was tested. During the initial screen, no marker from the panel was significantly (p < 0.05) associated with IBH. Five SNPs associated with IBH at p < 0.10 were therefore used for analysis of combined genotypes. Out of them, SNPs located in the genes coding for the CD14 receptor (CD14), interleukin 23 receptor (IL23R), thymic stromal lymphopoietin (TSLP) and transforming growth factor beta 3 (TGFB3) molecules were associated with IBH as parts of complex genotypes. These results are supported by similar associations and by expression data from different horse populations and from human studies.
- MeSH
- alergie genetika imunologie veterinární MeSH
- Ceratopogonidae imunologie patogenita MeSH
- dermatitida genetika veterinární MeSH
- hlavní histokompatibilní komplex genetika MeSH
- koně genetika imunologie MeSH
- kousnutí a bodnutí hmyzem genetika imunologie MeSH
- Simuliidae imunologie patogenita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Island MeSH
Anaplasma phagocytophilum is the aetiological agent of equine granulocytic anaplasmosis (EGA). The aim of this survey was to assess the prevalence of anti-A. phagocytophilum antibodies in the horse population of the Czech Republic (CZ) and to investigate possible links between seropositivity and the geographic origin, age, and/or sex of the tested horses. Antibodies against A. phagocytophilum were screened using an indirect fluorescent antibody method (IFA). Serum samples from 96 healthy horses from 8 localities (7 within the CZ and one control upland/tick-free locality in Ukraine) were examined. While the controls tested negative, the seroprevalence in the Czech localities was estimated at 73%, which suggests that subclinical EGA is common among horses in the CZ. Significant differences between seropositivity rates in individual stables were demonstrated (explicable by the different environment and/or management styles of the horses stabled therein). Horses in stables from which previous manifest cases had been reported tended to have higher average titres. Mares displayed a somewhat higher seropositivity rate than did stallions; age had no demonstrable effect upon the serological status of the examined horses.
- MeSH
- Anaplasma phagocytophilum imunologie MeSH
- ehrlichióza epidemiologie MeSH
- koně imunologie mikrobiologie MeSH
- nemoci koní epidemiologie MeSH
- séroepidemiologické studie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- antigeny CD27 * krev MeSH
- antigeny sacharidové asociované s nádorem * krev MeSH
- erytrocyty * imunologie MeSH
- isoantigeny * krev MeSH
- koně imunologie MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- monoklonální protilátky MeSH
- reprodukovatelnost výsledků MeSH
- sacharidové sekvence MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- dopisy MeSH
- komentáře MeSH
[Antibodies against Legionellae in domestic Aanimals]
- MeSH
- hospodářská zvířata imunologie mikrobiologie MeSH
- koně imunologie mikrobiologie MeSH
- králíci MeSH
- Legionella imunologie izolace a purifikace MeSH
- legionelóza diagnóza mikrobiologie veterinární MeSH
- prasata imunologie mikrobiologie MeSH
- sérologické testy MeSH
- skot MeSH
- tvorba protilátek MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- skot MeSH
- zvířata MeSH
- MeSH
- antisérum analýza MeSH
- časové faktory MeSH
- imunita získaná od matky MeSH
- imunodifuze MeSH
- infekce viry z čeledi Orthomyxoviridae imunologie MeSH
- injekce intramuskulární MeSH
- koně imunologie MeSH
- lidé MeSH
- Orthomyxoviridae imunologie MeSH
- reakce antigenu s protilátkou MeSH
- testy inhibice hemaglutinace MeSH
- tvorba protilátek MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- MeSH
- ektroforéza na škrobovém gelu metody využití MeSH
- elektroforéza sérových bílkovin * metody využití MeSH
- experimenty na lidech MeSH
- gama-globuliny analýza imunologie izolace a purifikace MeSH
- hematologické nádory * enzymologie imunologie krev MeSH
- koně imunologie krev MeSH
- králíci imunologie krev MeSH
- lidé MeSH
- precipitinové testy metody využití MeSH
- sérové globuliny * analýza imunologie izolace a purifikace MeSH
- sérum enzymologie MeSH
- statistika jako téma MeSH
- Check Tag
- králíci imunologie krev MeSH
- lidé MeSH
