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Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense

G. Krejčová, A. Danielová, P. Nedbalová, M. Kazek, L. Strych, G. Chawla, JM. Tennessen, J. Lieskovská, M. Jindra, T. Doležal, A. Bajgar,

. 2019 ; 8 (-) : . [pub] 20191014

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20023591

Grantová podpora
R35 GM119557 NIGMS NIH HHS - United States
Project 17-16406S Grantová Agentura České Republiky - International
Project 17-16406S Czech Science Foundation - International
R35 MIRA 1R35GM119557 NIGMS NIH HHS - United States

Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly conserved, the metabolic remodeling of insect macrophages remains poorly understood. Here, we used adults of the fruit fly Drosophila melanogaster to investigate the metabolic changes that occur in macrophages during the acute and resolution phases of Streptococcus-induced sepsis. Our studies revealed that orthologs of Hypoxia inducible factor 1α (HIF1α) and Lactate dehydrogenase (LDH) are required for macrophage activation, their bactericidal function, and resistance to infection, thus documenting the conservation of this cellular response between insects and mammals. Further, we show that macrophages employing aerobic glycolysis induce changes in systemic metabolism that are necessary to meet the biosynthetic and energetic demands of their function and resistance to bacterial infection.

Citace poskytuje Crossref.org

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